The p53 protein does not facilitate adenovirus type 5 replication in normal human cells.

TitleThe p53 protein does not facilitate adenovirus type 5 replication in normal human cells.
Publication TypeJournal Article
Year of Publication2013
AuthorsChahal, JS, Flint, SJ
JournalJ Virol
Volume87
Issue10
Pagination6044-6
Date Published2013 May
ISSN1098-5514
KeywordsAdenoviruses, Human, Cells, Cultured, Epithelial Cells, Gene Knockdown Techniques, Host-Pathogen Interactions, Humans, RNA Interference, Tumor Suppressor Protein p53, Virus Replication
Abstract

<p>Although several adenovirus type 5 (Ad5) proteins prevent deleterious consequences of activation of p53, it has been reported that viral replication proceeds more efficiently when human tumor cells produce wild-type compared to mutant p53. We have now exploited RNA interference and lentiviral vectors to achieve essentially complete knockdown of p53 in normal human cells: no effects on the kinetics or efficiency of viral gene expression or production of infectious particles were observed.</p>

DOI10.1128/JVI.00129-13
Alternate JournalJ. Virol.
PubMed ID23487462
PubMed Central IDPMC3648133
Grant ListR56 AI091785 / AI / NIAID NIH HHS / United States
R56AI1691785 / AI / NIAID NIH HHS / United States