Open LED Illuminator: A Simple and Inexpensive LED Illuminator for Fast Multicolor Particle Tracking in Neurons.

TitleOpen LED Illuminator: A Simple and Inexpensive LED Illuminator for Fast Multicolor Particle Tracking in Neurons.
Publication TypeJournal Article
Year of Publication2015
AuthorsBosse, JB, Tanneti, NS, Hogue, IB, Enquist, LW
JournalPLoS One
Volume10
Issue11
Paginatione0143547
Date Published2015
ISSN1932-6203
KeywordsAnimals, Humans, Lighting, Microscopy, Microscopy, Fluorescence, Neurons
Abstract

<p>Dual-color live cell fluorescence microscopy of fast intracellular trafficking processes, such as axonal transport, requires rapid switching of illumination channels. Typical broad-spectrum sources necessitate the use of mechanical filter switching, which introduces delays between acquisition of different fluorescence channels, impeding the interpretation and quantification of highly dynamic processes. Light Emitting Diodes (LEDs), however, allow modulation of excitation light in microseconds. Here we provide a step-by-step protocol to enable any scientist to build a research-grade LED illuminator for live cell microscopy, even without prior experience with electronics or optics. We quantify and compare components, discuss our design considerations, and demonstrate the performance of our LED illuminator by imaging axonal transport of herpes virus particles with high temporal resolution.</p>

DOI10.1371/journal.pone.0143547
Alternate JournalPLoS ONE
PubMed ID26600461
PubMed Central IDPMC4658086
Grant ListNS033506 / NS / NINDS NIH HHS / United States
NS060699 / NS / NINDS NIH HHS / United States