Myosin-dependent remodeling of adherens junctions protects junctions from Snail-dependent disassembly.

TitleMyosin-dependent remodeling of adherens junctions protects junctions from Snail-dependent disassembly.
Publication TypeJournal Article
Year of Publication2016
AuthorsWeng, M, Wieschaus, E
JournalJ Cell Biol
Volume212
Issue2
Pagination219-29
Date Published2016 Jan 18
ISSN1540-8140
KeywordsActomyosin, Adherens Junctions, Animals, Cadherins, Drosophila melanogaster, Drosophila Proteins, Epithelial-Mesenchymal Transition, Female, Gastrulation, Intercellular Junctions, Male, Mesoderm, Microscopy, Confocal, Myosin Type II, Transcription Factors
Abstract

<p>Although Snail is essential for disassembly of adherens junctions during epithelial-mesenchymal transitions (EMTs), loss of adherens junctions in Drosophila melanogaster gastrula is delayed until mesoderm is internalized, despite the early expression of Snail in that primordium. By combining live imaging and quantitative image analysis, we track the behavior of E-cadherin-rich junction clusters, demonstrating that in the early stages of gastrulation most subapical clusters in mesoderm not only persist, but move apically and enhance in density and total intensity. All three phenomena depend on myosin II and are temporally correlated with the pulses of actomyosin accumulation that drive initial cell shape changes during gastrulation. When contractile myosin is absent, the normal Snail expression in mesoderm, or ectopic Snail expression in ectoderm, is sufficient to drive early disassembly of junctions. In both cases, junctional disassembly can be blocked by simultaneous induction of myosin contractility. Our findings provide in vivo evidence for mechanosensitivity of cell-cell junctions and imply that myosin-mediated tension can prevent Snail-driven EMT.</p>

DOI10.1083/jcb.201508056
Alternate JournalJ. Cell Biol.
PubMed ID26754645
PubMed Central IDPMC4738385
Grant List5R37HD15587 / HD / NICHD NIH HHS / United States
/ / Howard Hughes Medical Institute / United States