Mitotic internalization of planar cell polarity proteins preserves tissue polarity.

TitleMitotic internalization of planar cell polarity proteins preserves tissue polarity.
Publication TypeJournal Article
Year of Publication2011
AuthorsDevenport, D, Oristian, D, Heller, E, Fuchs, E
JournalNat Cell Biol
Volume13
Issue8
Pagination893-902
Date Published2011 Jul 10
ISSN1476-4679
KeywordsAnimals, Cell Polarity, Cytokinesis, Endocytosis, Endosomes, Epidermis, Frizzled Receptors, Interphase, Mice, Mice, Transgenic, Mitosis, Mutation, Nerve Tissue Proteins, Receptors, G-Protein-Coupled, Stem Cells
Abstract

<p>Planar cell polarity (PCP) is the collective polarization of cells along the epithelial plane, a process best understood in the terminally differentiated Drosophila wing. Proliferative tissues such as mammalian skin also show PCP, but the mechanisms that preserve tissue polarity during proliferation are not understood. During mitosis, asymmetrically distributed PCP components risk mislocalization or unequal inheritance, which could have profound consequences for the long-range propagation of polarity. Here, we show that when mouse epidermal basal progenitors divide PCP components are selectively internalized into endosomes, which are inherited equally by daughter cells. Following mitosis, PCP proteins are recycled to the cell surface, where asymmetry is re-established by a process reliant on neighbouring PCP. A cytoplasmic dileucine motif governs mitotic internalization of atypical cadherin Celsr1, which recruits Vang2 and Fzd6 to endosomes. Moreover, embryos transgenic for a Celsr1 that cannot mitotically internalize exhibit perturbed hair-follicle angling, a hallmark of defective PCP. This underscores the physiological relevance and importance of this mechanism for regulating polarity during cell division.</p>

DOI10.1038/ncb2284
Alternate JournalNat. Cell Biol.
PubMed ID21743464
PubMed Central IDPMC3149741
Grant ListR37 AR027883-33 / AR / NIAMS NIH HHS / United States
R37 AR027883 / AR / NIAMS NIH HHS / United States
R37 AR027883-30S1 / AR / NIAMS NIH HHS / United States
R37 AR027883-31 / AR / NIAMS NIH HHS / United States
K99 AR057501 / AR / NIAMS NIH HHS / United States
AR027883-30S1 / AR / NIAMS NIH HHS / United States
R00 AR057501 / AR / NIAMS NIH HHS / United States
R37 AR027883-32 / AR / NIAMS NIH HHS / United States
AR027883-33 / AR / NIAMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
AR027883-32 / AR / NIAMS NIH HHS / United States
R01 AR050452-08 / AR / NIAMS NIH HHS / United States
R01 AR050452 / AR / NIAMS NIH HHS / United States
R01 AR050452-06A2 / AR / NIAMS NIH HHS / United States
R01 AR050452-06A2 / / None / None
R37 AR027883-30 / AR / NIAMS NIH HHS / United States
AR027883-31 / AR / NIAMS NIH HHS / United States
R01 AR050452-07 / AR / NIAMS NIH HHS / United States