Light-based feedback for controlling intracellular signaling dynamics.

TitleLight-based feedback for controlling intracellular signaling dynamics.
Publication TypeJournal Article
Year of Publication2011
AuthorsToettcher, JE, Gong, D, Lim, WA, Weiner, OD
JournalNat Methods
Volume8
Issue10
Pagination837-9
Date Published2011 Sep 11
ISSN1548-7105
KeywordsAnimals, Cell Membrane, Computational Biology, Feedback, Physiological, Humans, Light, Models, Biological, Phosphatidylinositol 3-Kinases, Protein Binding, Signal Transduction
Abstract

<p>The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.</p>

DOI10.1038/nmeth.1700
Alternate JournalNat. Methods
PubMed ID21909100
PubMed Central IDPMC3184382
Grant ListEY016546 / EY / NEI NIH HHS / United States
GM084040 / GM / NIGMS NIH HHS / United States
GM55040 / GM / NIGMS NIH HHS / United States
GM62583 / GM / NIGMS NIH HHS / United States
P50 GM081879 / GM / NIGMS NIH HHS / United States
P50 GM081879-01A2 / GM / NIGMS NIH HHS / United States
P50GM081879 / GM / NIGMS NIH HHS / United States
PN2 EY016546 / EY / NEI NIH HHS / United States
PN2 EY016546-06 / EY / NEI NIH HHS / United States
R01 GM055040 / GM / NIGMS NIH HHS / United States
R01 GM055040-12 / GM / NIGMS NIH HHS / United States
R01 GM062583 / GM / NIGMS NIH HHS / United States
R01 GM062583-08 / GM / NIGMS NIH HHS / United States
R01 GM084040 / GM / NIGMS NIH HHS / United States
R01 GM084040-04 / GM / NIGMS NIH HHS / United States
R01 GM096164 / GM / NIGMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States