Independent and coordinate trafficking of single Drosophila germ plasm mRNAs.

TitleIndependent and coordinate trafficking of single Drosophila germ plasm mRNAs.
Publication TypeJournal Article
Year of Publication2015
AuthorsLittle, SC, Sinsimer, KS, Lee, JJ, Wieschaus, EF, Gavis, ER
JournalNat Cell Biol
Volume17
Issue5
Pagination558-68
Date Published2015 May
ISSN1476-4679
KeywordsActive Transport, Cell Nucleus, Animals, Cell Nucleus, Cyclin B, Cytoplasmic Granules, Drosophila melanogaster, Drosophila Proteins, Microscopy, Video, Oocytes, Positive Transcriptional Elongation Factor B, RNA, Messenger, RNA-Binding Proteins, Transcription, Genetic
Abstract

<p>Messenger RNA localization is a conserved mechanism for spatial control of protein synthesis, with key roles in generating cellular and developmental asymmetry. Whereas different transcripts may be targeted to the same subcellular domain, the extent to which their localization is coordinated is unclear. Using quantitative single-molecule imaging, we analysed the assembly of Drosophila germ plasm mRNA granules inherited by nascent germ cells. We find that the germ-cell-destined transcripts nanos, cyclin B and polar granule component travel within the oocyte as ribonucleoprotein particles containing single mRNA molecules but co-assemble into multi-copy heterogeneous granules selectively at the posterior of the oocyte. The stoichiometry and dynamics of assembly indicate a defined stepwise sequence. Our data suggest that co-packaging of these transcripts ensures their effective segregation to germ cells. In contrast, compartmentalization of the germline determinant oskar mRNA into different granules limits its entry into germ cells. This exclusion is required for proper germline development.</p>

DOI10.1038/ncb3143
Alternate JournalNat. Cell Biol.
PubMed ID25848747
PubMed Central IDPMC4417036
Grant ListR01 GM067758 / GM / NIGMS NIH HHS / United States
R01GM067758 / GM / NIGMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States