Imaging TGFβ Signaling in Mouse Models of Cancer Metastasis.

TitleImaging TGFβ Signaling in Mouse Models of Cancer Metastasis.
Publication TypeJournal Article
Year of Publication2016
AuthorsKang, Y
JournalMethods Mol Biol
Volume1344
Pagination219-32
Date Published2016
ISSN1940-6029
KeywordsAnimals, Disease Models, Animal, Heterografts, Humans, Luminescent Measurements, Mice, Molecular Imaging, Neoplasm Metastasis, Neoplasms, Signal Transduction, Transforming Growth Factor beta
Abstract

Metastatic spread of cancer cells from the primary tumors to distant vital organs, such as lung, liver, brain, and bone, is responsible for the majority of cancer-related deaths. Development of metastatic lesions is critically dependent on the interaction of tumor cells with the stromal microenvironment. As a multifunctional paracrine signaling factor that is abundantly produced by both tumor and stromal cells, TGFβ has been well established as an important mediator of tumor-stromal interaction during cancer metastasis. Imaging the in vivo dynamic of TGFβ signaling activity during cancer metastasis is critical for understanding the pathogenesis of the disease, and for the development of effective anti-metastasis treatments. In this chapter, I describe several xenograft methods to introduce human breast cancer cells into nude mice in order to generate spontaneous and experimental metastases, as well as the luciferase-based bioluminescence imaging method for quantitative imaging analysis of TGFβ signaling in tumor cells during metastasis.

DOI10.1007/978-1-4939-2966-5_13
Alternate JournalMethods Mol. Biol.
PubMed ID26520127