Germ Cell-less Promotes Centrosome Segregation to Induce Germ Cell Formation.

TitleGerm Cell-less Promotes Centrosome Segregation to Induce Germ Cell Formation.
Publication TypeJournal Article
Year of Publication2017
AuthorsLerit, DA, Shebelut, CW, Lawlor, KJ, Rusan, NM, Gavis, ER, Schedl, P, Deshpande, G
JournalCell Rep
Volume18
Issue4
Pagination831-839
Date Published2017 Jan 24
ISSN2211-1247
Abstract

The primordial germ cells (PGCs) specified during embryogenesis serve as progenitors to the adult germline stem cells. In Drosophila, the proper specification and formation of PGCs require both centrosomes and germ plasm, which contains the germline determinants. Centrosomes are microtubule (MT)-organizing centers that ensure the faithful segregation of germ plasm into PGCs. To date, mechanisms that modulate centrosome behavior to engineer PGC development have remained elusive. Only one germ plasm component, Germ cell-less (Gcl), is known to play a role in PGC formation. Here, we show that Gcl engineers PGC formation by regulating centrosome dynamics. Loss of gcl leads to aberrant centrosome separation and elaboration of the astral MT network, resulting in inefficient germ plasm segregation and aborted PGC cellularization. Importantly, compromising centrosome separation alone is sufficient to mimic the gcl loss-of-function phenotypes. We conclude Gcl functions as a key regulator of centrosome separation required for proper PGC development.

DOI10.1016/j.celrep.2016.12.074
Alternate JournalCell Rep
PubMed ID28122234
Grant ListR01 GM110015 / GM / NIGMS NIH HHS / United States