Fluorescent Protein Approaches in Alpha Herpesvirus Research.

TitleFluorescent Protein Approaches in Alpha Herpesvirus Research.
Publication TypeJournal Article
Year of Publication2015
AuthorsHogue, IB, Bosse, JB, Engel, EA, Scherer, J, Hu, J-R, Del Rio, T, Enquist, LW
JournalViruses
Volume7
Issue11
Pagination5933-61
Date Published2015 Nov 19
ISSN1999-4915
KeywordsAlphaherpesvirinae, Biomedical Research, Host-Pathogen Interactions, Luminescent Proteins, Recombinant Fusion Proteins, Staining and Labeling, Virology
Abstract

In the nearly two decades since the popularization of green fluorescent protein (GFP), fluorescent protein-based methodologies have revolutionized molecular and cell biology, allowing us to literally see biological processes as never before. Naturally, this revolution has extended to virology in general, and to the study of alpha herpesviruses in particular. In this review, we provide a compendium of reported fluorescent protein fusions to herpes simplex virus 1 (HSV-1) and pseudorabies virus (PRV) structural proteins, discuss the underappreciated challenges of fluorescent protein-based approaches in the context of a replicating virus, and describe general strategies and best practices for creating new fluorescent fusions. We compare fluorescent protein methods to alternative approaches, and review two instructive examples of the caveats associated with fluorescent protein fusions, including describing several improved fluorescent capsid fusions in PRV. Finally, we present our future perspectives on the types of powerful experiments these tools now offer.

DOI10.3390/v7112915
Alternate JournalViruses
PubMed ID26610544
PubMed Central IDPMC4664988
Grant ListF32 GM112337 / GM / NIGMS NIH HHS / United States
F32 GM112337 / GM / NIGMS NIH HHS / United States
P40 RR018604 / RR / NCRR NIH HHS / United States
R01 NS033506 / NS / NINDS NIH HHS / United States
R01 NS033506 / NS / NINDS NIH HHS / United States
R01 NS060699 / NS / NINDS NIH HHS / United States
R01 NS060699 / NS / NINDS NIH HHS / United States