Fixed and live visualization of RNAs in Drosophila oocytes and embryos.

TitleFixed and live visualization of RNAs in Drosophila oocytes and embryos.
Publication TypeJournal Article
Year of Publication2016
AuthorsAbbaszadeh, EK, Gavis, ER
JournalMethods
Volume98
Pagination34-41
Date Published2016 Apr 01
ISSN1095-9130
KeywordsAnimals, Body Patterning, Drosophila melanogaster, Embryo, Nonmammalian, Female, Fixatives, Gene Expression Regulation, Developmental, In Situ Hybridization, Fluorescence, Oocytes, Recombinant Fusion Proteins, RNA, Messenger, Single Molecule Imaging, Staining and Labeling, Tissue Fixation
Abstract

<p>The ability to visualize RNA in situ is essential to dissect mechanisms for the temporal and spatial regulation of gene expression that drives development. Although considerable attention has been focused on transcriptional control, studies in model organisms like Drosophila have highlighted the importance of post-transcriptional mechanisms - most notably intracellular mRNA localization - in the formation and patterning of the body axes, specification of cell fates, and polarized cell functions. Our understanding of both types of regulation has been greatly advanced by technological innovations that enable a combination of highly quantitative and dynamic analysis of RNA. This review presents two methods, single molecule fluorescence in situ hybridization for high resolution quantitative RNA detection in fixed Drosophila oocytes and embryos and genetically encoded fluorescent RNA labeling for detection in live cells.</p>

DOI10.1016/j.ymeth.2016.01.018
Alternate JournalMethods
PubMed ID26827935
PubMed Central IDPMC4808400
Grant ListR01 GM067758 / GM / NIGMS NIH HHS / United States
R01GM067758 / GM / NIGMS NIH HHS / United States