Extracellular matrix structure governs invasion resistance in bacterial biofilms.

TitleExtracellular matrix structure governs invasion resistance in bacterial biofilms.
Publication TypeJournal Article
Year of Publication2015
AuthorsNadell, CD, Drescher, K, Wingreen, NS, Bassler, BL
JournalISME J
Volume9
Issue8
Pagination1700-9
Date Published2015 Aug
ISSN1751-7370
KeywordsBacterial Adhesion, Bacterial Proteins, Biofilms, Extracellular Matrix, Nuclear Proteins, Vibrio cholerae
Abstract

<p>Many bacteria are highly adapted for life in communities, or biofilms. A defining feature of biofilms is the production of extracellular matrix that binds cells together. The biofilm matrix provides numerous fitness benefits, including protection from environmental stresses and enhanced nutrient availability. Here we investigate defense against biofilm invasion using the model bacterium Vibrio cholerae. We demonstrate that immotile cells, including those identical to the biofilm resident strain, are completely excluded from entry into resident biofilms. Motile cells can colonize and grow on the biofilm exterior, but are readily removed by shear forces. Protection from invasion into the biofilm interior is mediated by the secreted protein RbmA, which binds mother-daughter cell pairs to each other and to polysaccharide components of the matrix. RbmA, and the invasion protection it confers, strongly localize to the cell lineages that produce it.</p>

DOI10.1038/ismej.2014.246
Alternate JournalISME J
PubMed ID25603396
PubMed Central IDPMC4511925
Grant List5R01GM065859 / GM / NIGMS NIH HHS / United States
R01 GM065859 / GM / NIGMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States