"Drosophila dosage compensation loci associate with a boundary forming insulator complex."

Title"Drosophila dosage compensation loci associate with a boundary forming insulator complex."
Publication TypeJournal Article
Year of Publication2017
AuthorsKaye, EG, Kurbidaeva, A, Wolle, D, Aoki, T, Schedl, P, Larschan, E
JournalMol Cell Biol
Date Published2017 Aug 07
ISSN1098-5549
Abstract

CES (chromatin entry sites) are 100-1,500 bp elements that recruit MSL (Male Specific Lethal) complexes to the X-chromosome to upregulate expression of X-linked genes in male flies. CES contain one or more ∼20 bp GA rich sequences called MREs (MSL recognition elements) that are critical for dosage compensation. Recent studies indicate that CES also correspond to boundaries of X-chromosomal TADs (topologically associated domains). Here we show that a ∼1,000 kDa complex called the LBC, which is required for the functioning of the Bithorax complex boundary Fab-7, interacts specifically with a special class of CES that contain multiple MREs. Mutations in the MRE sequences of three of these CES that disrupt function in vivo abrogate interactions with the LBC. Moreover, reducing the levels of two LBC components compromises MSL recruitment. Finally, we show that several of the CES that are physically linked to each other in vivo are LBC interactors.

DOI10.1128/MCB.00253-17
Alternate JournalMol. Cell. Biol.
PubMed ID28784719