Distinctive roles for periplasmic proteases in the maintenance of essential outer membrane protein assembly.

TitleDistinctive roles for periplasmic proteases in the maintenance of essential outer membrane protein assembly.
Publication TypeJournal Article
Year of Publication2017
AuthorsSoltes, GR, Martin, NR, Park, E, Sutterlin, HA, Silhavy, TJ
JournalJ Bacteriol
Date Published2017 Aug 07
ISSN1098-5530
Abstract

Outer membrane protein (OMP) biogenesis in Escherichia coli is a robust process essential to the life of the organism. It is catalyzed by the ß-barrel assembly machine (Bam) complex and a number of quality control factors, including periplasmic chaperones and proteases, maintain the integrity of this trafficking pathway. Little is known, however, about how periplasmic proteases recognize and degrade OMP substrates when assembly is compromised, or whether different proteases recognize the same substrate at distinct points in the assembly pathway. In this work, we use well-defined assembly-defective mutants of LptD, the essential lipopolysaccharide assembly translocon, to show that the periplasmic protease DegP degrades substrates with assembly defects that prevent or impair initial contact with Bam causing the mutant protein to accumulate in the periplasm. In contrast, another periplasmic protease, BepA, degrades an LptD mutant substrate that has engaged the Bam complex and formed a nearly complete barrel. Furthermore, we describe the role of the OM lipoprotein YcaL, a protease of heretofore-unknown function, in the degradation of an LptD substrate that has engaged the Bam complex but is stalled at an earlier step in the assembly process that is not accessible to BepA. Our results demonstrate that multiple periplasmic proteases monitor OMPs at distinct points in the assembly process.IMPORTANCE OMP assembly is catalyzed by the essential Bam complex and occurs in a cellular environment devoid of energy sources. Assembly intermediates that misfold can compromise this essential molecular machine. Here we demonstrate distinctive roles for three different periplasmic proteases that can clear OMP substrates with folding defects that compromise assembly at three different stages. These quality control factors help ensure the integrity of the permeability barrier that contributes to the intrinsic resistance of Gram-negative organisms to many antibiotics.

DOI10.1128/JB.00418-17
Alternate JournalJ. Bacteriol.
PubMed ID28784813