CqsA-CqsS quorum-sensing signal-receptor specificity in Photobacterium angustum.

TitleCqsA-CqsS quorum-sensing signal-receptor specificity in Photobacterium angustum.
Publication TypeJournal Article
Year of Publication2014
AuthorsKe, X, Miller, LC, Ng, W-L, Bassler, BL
JournalMol Microbiol
Volume91
Issue4
Pagination821-33
Date Published2014 Feb
ISSN1365-2958
KeywordsMembrane Proteins, Pheromones, Photobacterium, Quorum Sensing, Substrate Specificity
Abstract

<p>Quorum sensing (QS) is a process of bacterial cell-cell communication that relies on the production, detection and population-wide response to extracellular signal molecules called autoinducers. The QS system commonly found in vibrios and photobacteria consists of the CqsA synthase/CqsS receptor pair. Vibrio cholerae CqsA/S synthesizes and detects (S)-3-hydroxytridecan-4-one (C10-CAI-1), whereas Vibrio harveyi produces and detects a distinct but similar molecule, (Z)-3-aminoundec-2-en-4-one (Ea-C8-CAI-1). To understand the signalling properties of the larger family of CqsA-CqsS pairs, here, we characterize the Photobacterium angustum CqsA/S system. Many photobacterial cqsA genes harbour a conserved frameshift mutation that abolishes CAI-1 production. By contrast, their cqsS genes are intact. Correcting the P. angustum cqsA reading frame restores production of a mixture of CAI-1 moieties, including C8-CAI-1, C10-CAI-1, Ea-C8-CAI-1 and Ea-C10-CAI-1. This signal production profile matches the P. angustum CqsS receptor ligand-detection capability. The receptor exhibits a preference for molecules with 10-carbon tails, and the CqsS Ser(168) residue governs this preference. P. angustum can overcome the cqsA frameshift to produce CAI-1 under particular limiting growth conditions presumably through a ribosome slippage mechanism. Thus, we propose that P. angustum uses CAI-1 signalling for adaptation to stressful environments.</p>

DOI10.1111/mmi.12502
Alternate JournalMol. Microbiol.
PubMed ID24372841
PubMed Central IDPMC3959898
Grant List5R01GM065859 / GM / NIGMS NIH HHS / United States
R01 GM065859 / GM / NIGMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
/ / Howard Hughes Medical Institute / United States