Cellular resolution functional imaging in behaving rats using voluntary head restraint.

TitleCellular resolution functional imaging in behaving rats using voluntary head restraint.
Publication TypeJournal Article
Year of Publication2013
AuthorsScott, BB, Brody, CD, Tank, DW
JournalNeuron
Volume80
Issue2
Pagination371-84
Date Published2013 Oct 16
ISSN1097-4199
KeywordsAnimals, Conditioning, Operant, Evoked Potentials, Visual, Functional Neuroimaging, Head, Immobilization, Male, Molecular Imaging, Rats, Visual Cortex
Abstract

<p>High-throughput operant conditioning systems for rodents provide efficient training on sophisticated behavioral tasks. Combining these systems with technologies for cellular resolution functional imaging would provide a powerful approach to study neural dynamics during behavior. Here we describe an integrated two-photon microscope and behavioral apparatus that allows cellular resolution functional imaging of cortical regions during epochs of voluntary head restraint. Rats were trained to initiate periods of restraint up to 8 s in duration, which provided the mechanical stability necessary for in vivo imaging while allowing free movement between behavioral trials. A mechanical registration system repositioned the head to within a few microns, allowing the same neuronal populations to be imaged on each trial. In proof-of-principle experiments, calcium-dependent fluorescence transients were recorded from GCaMP-labeled cortical neurons. In contrast to previous methods for head restraint, this system can be incorporated into high-throughput operant conditioning systems.</p>

DOI10.1016/j.neuron.2013.08.002
Alternate JournalNeuron
PubMed ID24055015
PubMed Central IDPMC4068252
Grant ListF32 NS078913 / NS / NINDS NIH HHS / United States
R21 NS082956 / NS / NINDS NIH HHS / United States
R21NS082956 / NS / NINDS NIH HHS / United States
RC1 NS068148 / NS / NINDS NIH HHS / United States
RC1NS068148 / NS / NINDS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States