The cell biology of againg

TitleThe cell biology of againg
Publication TypeJournal Article
Year of Publication2015
AuthorsHahm, J-H, Kim, S, DiLoreto, R, Shi, C, Lee, S-JV, Murphy, CT, Nam, HGil
JournalNat Commun
Volume6
Pagination8919
Date Published2015 Nov 20
ISSN2041-1723
KeywordsAnimals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Female, Insulin, Insulin-Like Growth Factor I, Longevity, Male, Mutation, Receptor, Insulin, Signal Transduction
Abstract

Ageing is marked by physical decline. Caenorhabditis elegans is a valuable model for identifying genetic regulatory mechanisms of ageing and longevity. Here we report a simple method to assess C. elegans' maximum physical ability based on the worms' maximum movement velocity. We show maximum velocity declines with age, correlates well with longevity, accurately reports movement ability and, if measured in mid-adulthood, is predictive of maximal lifespan. Contrary to recent findings, we observe that maximum velocity of worm with mutations in daf-2(e1370) insulin/IGF-1 signalling scales with lifespan. Because of increased odorant receptor expression, daf-2(e1370) mutants prefer food over exploration, causing previous on-food motility assays to underestimate movement ability and, thus, worm health. Finally, a disease-burden analysis of published data reveals that the daf-2(e1370) mutation improves quality of life, and therefore combines lifespan extension with various signs of an increased healthspan.

DOI10.1038/ncomms9919
Alternate JournalNat Commun
PubMed ID26586186
PubMed Central IDPMC4656132
Grant ListT32 GM007388 / GM / NIGMS NIH HHS / United States
5T32GM007388-38 / GM / NIGMS NIH HHS / United States