Biofilm streamers cause catastrophic disruption of flow with consequences for environmental and medical systems.

TitleBiofilm streamers cause catastrophic disruption of flow with consequences for environmental and medical systems.
Publication TypeJournal Article
Year of Publication2013
AuthorsDrescher, K, Shen, Y, Bassler, BL, Stone, HA
JournalProc Natl Acad Sci U S A
Volume110
Issue11
Pagination4345-50
Date Published2013 Mar 12
ISSN1091-6490
KeywordsBiofilms, Equipment Contamination, Membranes, Artificial, Porosity, Pseudomonas aeruginosa
Abstract

<p>Biofilms are antibiotic-resistant, sessile bacterial communities that occupy most moist surfaces on Earth and cause chronic and medical device-associated infections. Despite their importance, basic information about biofilm dynamics in common ecological environments is lacking. Here, we demonstrate that flow through soil-like porous materials, industrial filters, and medical stents dramatically modifies the morphology of Pseudomonas aeruginosa biofilms to form 3D streamers, which, over time, bridge the spaces between obstacles and corners in nonuniform environments. We discovered that accumulation of surface-attached biofilm has little effect on flow through such environments, whereas biofilm streamers cause sudden and rapid clogging. We demonstrate that flow-induced shedding of extracellular matrix from surface-attached biofilms generates a sieve-like network that captures cells and other biomass, which add to the existing network, causing exponentially fast clogging independent of growth. These results suggest that biofilm streamers are ubiquitous in nature and strongly affect flow through porous materials in environmental, industrial, and medical systems.</p>

DOI10.1073/pnas.1300321110
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID23401501
PubMed Central IDPMC3600445
Grant List5R01GM065859 / GM / NIGMS NIH HHS / United States
R01 GM065859 / GM / NIGMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States