bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring.

Titlebicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring.
Publication TypeJournal Article
Year of Publication2016
AuthorsTrovisco, V, Belaya, K, Nashchekin, D, Irion, U, Sirinakis, G, Butler, R, Lee, JJ, Gavis, ER, St Johnston, D
JournalElife
Volume5
Date Published2016 10 28
ISSN2050-084X
Abstract

bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot(2A2), which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110-120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.

DOI10.7554/eLife.17537
Alternate JournalElife
PubMed ID27791980
PubMed Central IDPMC5125753
Grant ListR01 GM067758 / GM / NIGMS NIH HHS / United States