Architectural transitions in Vibrio cholerae biofilms at single-cell resolution.

TitleArchitectural transitions in Vibrio cholerae biofilms at single-cell resolution.
Publication TypeJournal Article
Year of Publication2016
AuthorsDrescher, K, Dunkel, J, Nadell, CD, van Teeffelen, S, Grnja, I, Wingreen, NS, Stone, HA, Bassler, BL
JournalProc Natl Acad Sci U S A
Volume113
Issue14
PaginationE2066-72
Date Published2016 Apr 5
ISSN0027-8424
Abstract

<p>Many bacterial species colonize surfaces and form dense 3D structures, known as biofilms, which are highly tolerant to antibiotics and constitute one of the major forms of bacterial biomass on Earth. Bacterial biofilms display remarkable changes during their development from initial attachment to maturity, yet the cellular architecture that gives rise to collective biofilm morphology during growth is largely unknown. Here, we use high-resolution optical microscopy to image all individual cells in Vibrio cholerae biofilms at different stages of development, including colonies that range in size from 2 to 4,500 cells. From these data, we extracted the precise 3D cellular arrangements, cell shapes, sizes, and global morphological features during biofilm growth on submerged glass substrates under flow. We discovered several critical transitions of the internal and external biofilm architectures that separate the major phases of V. cholerae biofilm growth. Optical imaging of biofilms with single-cell resolution provides a new window into biofilm formation that will prove invaluable to understanding the mechanics underlying biofilm development.</p>

DOI10.1073/pnas.1601702113
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID26933214
PubMed Central IDPMC4833255
Grant ListR37 GM065859 / GM / NIGMS NIH HHS / United States