Bassler Lab Publications

2013

van Kessel JC, Ulrich LE, Zhulin IB, and Bassler BL (2013) Analysis of Activator and Repressor Functions Reveals the Requirements for Transcriptional Control by LuxR, the Master Regulator of Quorum Sensing in Vibrio harveyi. mBio, in press.

Shao Y*, Feng L*, Rutherford ST, Papenfort K, and Bassler BL (2013) Functional determinants of the quorum-sensing non-coding RNAs and their roles in target regulation. EMBO J., in press. (* co-firstauthors). 111.

Bassler B, Vogel J. (2013) Bacterial regulatory mechanisms: the gene and beyond.  Curr Opin Microbiol Epub 23 April. ISSN 1369-5274, 10.1016/j.mib.2013.04.001

Drescher K, Shen Y, Bassler BL, Stone HA. (2013) Biofilm streamers cause catastrophic disruption of flow with consequences for environmental and medical systems. Proc Natl Acad Sci U S A. 110:4345-4350 PMCID:PMC3600445

Nadell CD, Bucci V, Drescher K, Levin SA, Bassler BL, Xavier JB. (2013) Cutting through the complexity of cell collectives. Proc Royal Society. 280 20122770;doi:10. 1098/rspb.2012.2770.

van Kessel JC, Rutherford ST, Shao Y, Utria AF, Bassler BL. (2013) Individual and combined roles of the master regulators AphA and LuxR in control of the Vibrio harveyi quorum-sensing regulon. J Bacteriol 195:436-443.

2012

Perez LJ, Ng WL, Marano P, Brook K, Bassler BL, Semmelhack MF (2012) Role of the CAI-1 fatty acid tail in the Vibrio cholerae quorum sensing response.  J Med Chem 55:9669-9681

Rutherford ST, Bassler BL. (2012) Bacterial quorum sensing: its role in virulence and possibilities for its control.  In Cold Spring Harbor Perspectives. Bacterial Pathogens Eds Maloy S and Cossart P. Cold Spring Harbor Press

Ng WL, Perez L, Cong J, Semmelhack MF, Bassler BL. (2012) Broad spectrum pro-quorum-sensing molecules as inhibitors of virulence in vibrios.  PLoS Pathog Jun; 8(6):e1002767. Epub Jun 28. PMCID: PMC3386246

Bassler BL, (2012) Microbes as Menaces, Mates, and Marvels.  In Daedalus.  Eds. Barenbaum M and Meinwald G.  American Academy of Arts and Sciences Press. Cambridge MA Vol. 141. No 3 pp 67-76

Wei Y, Ng WL, Cong J, Bassler BL (2012) Ligand and antagonist driven regulation of the Vibrio cholerae quorum-sensing receptor CqsS. Mol Microbiol  83:1095-1108. PMCID: PMC3310172

Shao Y, Bassler BL (2012) Quorum-sensing non-coding small RNAs use unique pairing regions to differentially control mRNA targets. Mol Microbiol  83: 599-611 PMCID: PMC3262071

2011

Bolitho ME, Perez LJ, Koch MJ, Ng WL, Bassler BL, Semmelhack MF (2011) Small molecule probes of the receptor binding site in the Vibrio cholerae CAI-1 quorum sensing circuit. Bioorg Med Chem 2011 Nov 15;19(22):6906-18.

Nadell CD and Bassler BL (2011) A fitness trade-off between local competition and dispersal in Vibrio cholerae biofilmsProc Natl Acad Sci, USA. 108:14181-14185 PMCID: PMC3161532

Wang Y, Tu KC, Ong NP, Bassler BL, and Wingreen NS (2011) Protein level fluctuation correlation at the microcolony level and its application to the Vibrio harveyi quorum-sensing circuit. Biophys J Jun 22;100(12):3045-53.

Stauff  DL and BL Bassler (2011) Quorum Sensing in Chromobacterium violaceum: DNA Recognition and Gene Regulation by the CviR Receptor. J Bacteriol 193:3871-3878.

Teng, S-W, Schaffer JN., Tu KC, Mehta, P, Lu W, Ong, NP, Bassler BL, and NS Wingreen (2011) Active regulation of receptor ratios controls Integration of quorum-sensing signals in Vibrio harveyi. Mol Syst Biol 7:491. PMCID: PMC3130561.

Chen G, Swem LR, Swem DL, Stauff DL, O'Loughlin CT, Jeffrey PD, Bassler BL, Hughson FM. (2011) A strategy for antagonizing quorum sensing. Mol Cell 42: 199-209.

Ng WL, Perez LJ, Wei Y, Kraml C, Semmelhack MF, Bassler BL. (2011) Signal production and detection specificity in Vibrio CqsA/CqsS quorum-sensing systems. Mol Microbiol. 79: 1407-1417.

Wei Y, Perez LJ, Ng WL, Semmelhack MF, Bassler BL (2011) Mechanism of Vibrio cholerae autoinducer-1 Biosynthesis. ACS Chem Biol. 6: 356-365.

Rutherford ST, van Kessel JC, Shao Y, Bassler BL (2011) AphA and LuxR/HapR reciprocally control quorum sensing in vibrios. Genes Dev 25: 397-408.

2010

Bassler BL (2010) Cell to Cell Communication. Proc Am Philos Soc 154:307-314

Bassler BL (2010) Small cells--big future. Mol Biol Cell 21: 3786-3787.

Waters CM, Wu JT, Ramsey ME, Harris RC, Bassler BL (2010) Quorum sensing controls type three secretion in Vibrio harveyi through repression of ExsA. Appl Environ Microbiol 76: 4996-5004.

Ng WL, Wei Y, Perez LJ, Cong J, Long T, Koch M, Semmelhack MF, Wingreen NS, Bassler BL. (2010) Probing bacterial transmembrane histidine kinase receptor-ligand interactions with natural and synthetic molecules. Proc Natl Acad Sci. 107: 5575-5580.

Teng SW, Wang Y, Tu KC, Long T, Mehta P, Wingreen NS, Bassler BL, Ong NP. (2010) Measurement of the copy number of the master quorum-sensing regulator of a bacterial cell. Biophys J 98: 2024-2031.

Tu KC, Long T, Svenningsen SL, Wingreen NS, Bassler BL. (2010) Negative feedback loops involving small regulatory RNAs precisely control the Vibrio harveyi quorum-sensing response. Mol Cell 37: 567-579.

2009

Mehta P, Goyal S, Long T, Bassler BL, Wingreen NS (2009) Information processing and signal integration in bacterial quorum sensing. Mol Syst Biol 5: 325.

Kelly RC, Bolitho ME, Higgins DA, Lu W, Ng WL, Jeffrey PD, Rabinowitz JD, Semmelhack MF, Hughson FM, Bassler BL. (2009) The Vibrio cholerae quorum-sensing autoinducer CAI-1: analysis of the biosynthetic enzyme CqsA. Nat Chem Biol 5: 891-895.

Ng WL, Bassler BL (2009) Bacterial quorum-sensing network architectures. Annu Rev Genetics 43: 197-222.

Swem LR, Swem DL, O'Loughlin CT, Gatmaitan R, Zhao B, Ulrich SM, Bassler BL (2009) A quorum-sensing antagonist targets both membrane-bound and cytoplasmic receptors and controls bacterial pathogenicity. Mol Cell 35: 143-153.

Long T, Tu KC, Wang Y, Mehta P, Ong NP, Bassler BL, Wingreen NS (2009) Quantifying the integration of quorum-sensing signals with single-cell resolution. PLoS Biol 7: e68

Svenningsen SL, Tu KC, Bassler BL (2009) Gene dosage compensation calibrates four regulatory RNAs to control Vibrio cholerae quorum sensing. EMBO J 28: 429-439.

Hammer BK, Bassler BL (2009) Distinct sensory pathways in Vibrio cholerae El Tor and Classical biotypes modulate c-di-GMP levels to control biofilm formation. J Bacteriol 191: 169-177.

Hickson J, Yamada SD, Berger J, Alverdy J, O'Keefe J, Bassler BL and Rinker-Schaeffer C (2009) Societal interaction in ovarian cancer matastasis: a quorum-sensing hypothesis.  Clin Exp Metastasis 26: 67-76

2008

Tu KC, Waters CM, Svenningsen SL, Bassler BL (2008) A small-RNA-mediated negative feedback loop controls quorum-sensing dynamics in Vibrio harveyi. Mol Microbiol 70: 896-907.

Nadell CD, Bassler BL, Levin SA (2008) Observing bacteria through the lens of social evolution. J Biol 7: 27.

Pompeani AJ, Irgon JJ, Berger MF, Bulyk ML, Wingreen NS, Bassler BL (2008) The Vibrio harveyi master quorum-sensing regulator, LuxR, a TetR-type protein is both an activator and a repressor: DNA recognition and binding specificity at target promoters. Mol Microbiol 70: 76-88.

Swem LR, Swem DL, Wingreen NS, Bassler BL (2008) Deducing receptor signaling parameters from in vivo analysis: LuxN/AI-1 quorum sensing in Vibrio harveyi. Cell 134: 461-473.

Waters CM, Lu W, Rabinowitz JD, Bassler BL (2008) Quorum sensing controls biofilm formation in Vibrio cholerae through modulation of cyclic di-GMP levels and repression of vpsT. J Bacteriol 190: 2527-2536.

Nackerdien, Keynan ZA, Bassler BL, Lederberg J and Thaler DS (2008) Quorum sensing influences Vibrio harveyi growth rates in a manner not fully accounted for by the marker effect of bioluminescence. PLoS ONE 3:1-9. PMCID:PMC2249925.

Hammer BK and Bassler BL (2008) Signal integration in the Vibrio harveyi and Vibrio cholerae quorum sensing circuits.  In: Chemical Communication Among Microbes, eds. Bassler BL and Winans SC, American Society for Microbiology, Washington DC pp 323-332

Winans SC and Bassler BL (Eds) (2008) Chemical Communication Among Bacteria.  American Society for Microbiology, Washington DC

Svenningsen SL, Waters CM, Bassler BL (2008) A negative feedback loop involving small RNAs accelerates Vibrio cholerae's transition out of quorum-sensing mode. Genes Dev 22: 226-238.

2007

Bassler BL. (2007) Playing the game with nature. Biotechniques. 42: 123.

Higgins DA, Pomianek ME, Kraml CM, Taylor RK, Semmelhack MF and Bassler BL (2007) The major Vibrio cholerae autoinducer and its role in virulence factor productionNature 450:883-886

Rader BA, Campagna SR, Semmelhack MF, Bassler BL, Guillemin K.. (2007) The quorum sensing molecule AI-2 regulates motility and flagellar morphogenesis in Helicobacter pylori. J Bacteriol 189: 6109-6117

Hammer BK and Bassler BL (2007) Regulatory small RNAs circumvent the conventional quorum sensing pathway in pandemic Vibrio cholerae. Proc Natl Acad Sci USA 104: 11145-11149

Xavier KB, Miller ST, Lu W, Kim JH, Rabinowitz J, Pelczer I, Semmelhack MF, Bassler BL (2007) Phosphorylation and processing of the quorum-sensing molecule autoinducer-2 in enteric bacteria. ACS Chem Biol 2: 128-136.

Tu KC, Bassler BL (2007) Multiple small RNAs act additively to integrate sensory information and control quorum sensing in Vibrio harveyi. Genes Dev 21: 221-233. 

Lenz DH and Bassler BL (2007) The small nucleoid protein Fis is involved in Vibrio cholerae quorum sensing. Mol Microbiol, 63:859-871.

2006

Walsh, CT, BL Bassler, CF Nathan, TF O'Brien, M Riley, RJ White, GD Wright. (2006) Treating Infectious Diseases in a Microbial World National Research Council. National Academy of Science. USA

Waters CM and Bassler BL. (2006) The Vibrio harveyi quorum-sensing system uses shared regulatory components to discriminate between multiple autoinducers. Genes Dev 20: 2754-2767.

Timmen, M, BL Bassler, and K Jung (2006) AI - 1 Influences the kinase activity but not the phosphatase activity of LuxN of Vibrio harveyi. J Biol Chem, 281: 2439 - 24404.

Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM. (2006) Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing. Cell 126: 1095-1108.

Rickard, AH, RJ Palmer Jr, DS Blehert, SR Campagna, MF Semmelhack, PG Egland, BL Bassler, and PE Kolenbrander (2006) Autoinducer -  2: a concentration - dependent signal for mutualistic bacterial biofilm growth. Mol Microbiol, 60:1446 - 1456.

Bassler BL, Losick R. (2006) Bacterially speaking. Cell 125: 237-246.

Camilli A, Bassler BL. (2006) Bacterial small-molecule signaling pathways. Science 311: 1113-1116. 

Bassler BL. (2006) Cell-to-cell communication in bacteria: a chemical discourse. Harvey Lect 2004-2005 100: 123-142.

2005

Lenz DH, Miller MB, Zhu J, Kulkarni RV and Bassler BL (2005) CsrA and three redunCsrA and three redundant small RNAs regulate quorum sensing in Vibrio cholerae. Mol Microbiol 58: 1186-1202. 

Xavier KB, Bassler BL. (2005) Interference with AI-2-mediated bacterial cell-cell communication. Nature 437: 750-753.

Waters CM, Bassler BL. (2005) Quorum sensing: cell-to-cell communication in bacteria. Annu Rev Cell Dev Biol 21: 319-346. 

Neiditch MB, Federle MJ, Miller ST, Bassler BL and Hughson FM (2005) Regulation of LuxPQ receptor activity by the quorum-sensing signal autoinducer-2. Mol Cell 18: 507-518. 

Ulrich DL, Kojetin D, Bassler BL, Cavanagh J, Loria JP. (2005) Solution structure and dynamics of LuxU from Vibrio harveyi, a phosphotransferase protein involved in bacterial quorum sensing. J Mol Biol 347: 297-307. 

Semmelhack MF, Campagna SR, Federle MJ and Bassler BL (2005) An expeditious synthesis of DPD and boron binding studies. Org Lett 7: 569-572. 

Xavier KB, Bassler BL (2005) Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli. J Bacteriol 187: 238-248. 

2004

Ulrich DL, Thompson R, Bassler B, Cavanagh J and Loria JP (2004) 1H, 15N, and 13C chemical shift assignments of the Vibrio harveyi histidine phosphotransferase protein LuxU. J Biomol NMR 29: 551-552. 

Henke JM and Bassler BL (2004) Quorum sensing regulates type III secretion in Vibrio harveyi and Vibrio parahaemolyticus. J Bacteriol 186: 3794-3805. 

Henke JM and Bassler BL (2004) Three parallel quorum-sensing systems regulate gene expression in Vibrio harveyi. J Bacteriol 186: 6902-6914. 

Henke JM and Bassler BL (2004) Bacterial social engagements. Trends Cell Biol 14: 648-656. 

Semmelhack MF, Campagna SR, Hwa C, Federle MJ and Bassler BL (2004) Boron binding with the quorum sensing signal AI-2 and analogues. Org Lett 6: 2635-2637. 

Miller ST, Xavier KB, Campagna SR, Taga ME, Semmelhack MF, et al. (2004) Salmonella typhimurium recognizes a chemically distinct form of the bacterial quorum-sensing signal AI-2. Mol Cell 15: 677-687. 

Lenz DH, Mok KC, Lilley BN, Kulkarni RV, Wingreen NS, et al. (2004) The small RNA chaperone Hfq and multiple small RNAs control quorum sensing in Vibrio harveyi and Vibrio cholerae. Cell 118: 69-82. 

Henke, JM and BL Bassler (2004) Quorum sensing controls type III secretion in Vibrio harveyi. J. Bacteriol.186:3794 - 3805. PMCID: PMC419960.

2003

Taga ME and Bassler BL (2003) Chemical communication among bacteria. Proc Natl Acad Sci USA 100 Suppl 2: 14549-14554. 

Zhao G, Wan W, Mansouri S, Alfaro JF, Bassler BL, et al. (2003) Chemical synthesis of S-ribosyl-L-homocysteine and activity assay as a LuxS substrate. Bioorg Med Chem Lett 13: 3897-3900. 

Federle MJ and Bassler BL (2003) Interspecies communication in bacteria. J Clin Invest 112: 1291-1299. 

Taga ME, Miller ST and Bassler BL (2003) Lsr-mediated transport and processing of AI-2 in Salmonella typhimurium. Mol Microbiol 50: 1411-1427. 

Hammer BK and Bassler BL (2003) Quorum sensing controls biofilm formation in Vibrio cholerae. Mol Microbiol 50: 101-104. 

Xavier KB and Bassler BL (2003) LuxS quorum sensing: more than just a numbers game. Curr Opin Microbiol 6: 191-197. 

Mok KC, Wingreen NS and Bassler BL (2003) Vibrio harveyi quorum sensing: a coincidence detector for two autoinducers controls gene expression. Embo J 22: 870-881. 

2002

Mok, KC and BL Bassler. (2002) Two - component control of quorum sensing in Gram negative bacteria. In: Histidine Kinases in Signal Transduction , eds. M. Inouye and R. Dutta, Academic Press, London, pp. 313 - 340.

Miller MB, Skorupski K, Lenz DH, Taylor RK and Bassler BL (2002) Parallel quorum sensing systems converge to regulate virulence in Vibrio cholerae. Cell 110: 303-314. 

Bassler BL (2002) Small talk. Cell-to-cell communication in bacteria. Cell 109: 421-424. 

Zhu J, Miller MB, Vance RE, Dziejman M, Bassler BL, et al. (2002) Quorum-sensing regulators control virulence gene expression in Vibrio cholerae. Proc Natl Acad Sci USA 99: 3129-3134. 

Chen X, Schauder S, Potier N, Van Dorsselaer A, Pelczer I, et al. (2002) Structural identification of a bacterial quorum-sensing signal containing boron. Nature 415: 545-549. 

Winans SC and Bassler BL (2002) Mob psychology. J Bacteriol 184: 873-883. 

2001

Taga ME, Semmelhack JL and Bassler BL (2001) The LuxS-dependent autoinducer AI-2 controls the expression of an ABC transporter that functions in AI-2 uptake in Salmonella typhimurium. Mol Microbiol 42: 777-793. 

Schauder S, Shokat K, Surette MG and Bassler BL (2001) The LuxS family of bacterial autoinducers: biosynthesis of a novel quorum-sensing signal molecule. Mol Microbiol 41: 463-476. 

Schauder S and Bassler BL (2001) The languages of bacteria. Genes Dev 15: 1468-1480. 

Miller MB and Bassler BL (2001) Quorum sensing in bacteria. Annu Rev Microbiol 55: 165-199. 

Bassler, BL (2001) Tiny conspiracies: cell-to-cell communication allows bacteria to coordinate their activity. Natural History Magazine 110:16-22.

Miller, MB and BL Bassler. (2001) Quorum Sensing. In: The Prokaryotes. M. Dworkin (ed) Electronic Format

2000

Joyce EA, Bassler BL and Wright A (2000) Evidence for a signaling system in Helicobacter pylori: detection of a luxS-encoded autoinducer. J Bacteriol 182: 3638-3643. 

Lilley BN and Bassler BL (2000) Regulation of quorum sensing in Vibrio harveyi by LuxO and sigma-54. Mol Microbiol 36: 940-954. 

Freeman JA, Lilley BN and Bassler BL (2000) A genetic analysis of the functions of LuxN: a two-component hybrid sensor kinase that regulates quorum sensing in Vibrio harveyi. Mol Microbiol 35: 139-149. 

1999

Bassler BL (1999) How bacteria talk to each other: regulation of gene expression by quorum sensing. Curr Opin Microbiol 2: 582-587.

Surette MG, Miller MB and Bassler BL (1999) Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production. Proc Natl Acad Sci USA 96: 1639-1644. 

Freeman JA and Bassler BL (1999) Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. J Bacteriol 181: 899-906. 

Freeman JA and Bassler BL (1999) A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. Mol Microbiol 31: 665-677. 

Surette MG and Bassler BL (1999) Regulation of autoinducer production in Salmonella typhimurium. Mol Microbiol 31: 585-595. 

Bassler, BL (1999) A multi - channel two-component signalling relay control s quorum sensing in Vibrio harveyi. In: Bacterial Cell-
Cell Signalling , eds. G. Dunny and S. Winans. American Society for Microbiology, Washington, DC p. 259 - 273.

1987-1998

Surette MG and Bassler BL (1998) Quorum sensing in Escherichia coli and Salmonella typhimurium. Proc Natl Acad Sci USA 95: 7046-7050. 

Bassler BL, Greenberg EP and Stevens AM (1997) Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi. J Bacteriol 179: 4043-4045. 

Bassler, BL, and MR Silverman. 1994 Intercellular communication in marine Vibrio: Density-dependent regulation of the expression of bioluminescence. In:Two Component Signal Transduction, eds. J.A. Hoch and T.J. Silhavy, American Society for Microbiology, Washington, DC, pp. 431-445.

Bassler BL, Wright M and Silverman MR (1994) Multiple signalling systems controlling expression of luminescence in Vibrio harveyi: sequence and function of genes encoding a second sensory pathway. Mol Microbiol 13: 273-286. 

Bassler BL, Wright M and Silverman MR (1994) Sequence and function of LuxO, a negative regulator of luminescence in Vibrio harveyi. Mol Microbiol 12: 403-412. 

Bassler BL, Wright M, Showalter RE and Silverman MR (1993) Intercellular signalling in Vibrio harveyi: sequence and function of genes regulating expression of luminescence. Mol Microbiol 9: 773-786. 

Yu C, Bassler BL and Roseman S (1993) Chemotaxis of the marine bacterium Vibrio furnissii to sugars. A potential mechanism for initiating the chitin catabolic cascade. J Biol Chem 268: 9405-9409. 

Bassler BL, Yu C, Lee YC and Roseman S (1991) Chitin utilization by marine bacteria. Degradation and catabolism of chitin oligosaccharides by Vibrio furnissii. J Biol Chem 266: 24276-24286. 

Bassler, B., P. Gibbons, C. Yu, and S. Roseman (1991) Chitin utilization by marine bacteria. Chemotaxis to chitin oligosaccharides by Vibrio furnissii. J Biol Chem 266: 24268-24275. 

Yu, C., A. Lee, B. Bassler, and S. Roseman (1991) Chitin utilization by marine bacteria. A physiological function for bacterial adhesion to immobilized carbohydrates. J Biol Chem 266: 24260-24267. 

Bassler B, Gibbons P and Roseman S (1989) Chemotaxis to chitin oligosaccharides by Vibrio furnissii, a chitinivorous marine bacterium. Biochem Biophys Res Commun 161: 1172-1176. 

Hallenbeck PC, Vimr ER, Yu F, Bassler B and Troy FA (1987) Purification and properties of a bacteriophage-induced endo-N-acetylneuraminidase specific for poly-alpha-2,8-sialosyl carbohydrate units. J Biol Chem 262: 3553-3561.