Faculty & Research
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Molecular Biology Faculty

Mohamed S. Donia

assistant Professor of Molecular Biology

Mohamed Donia

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lab Donia Lab
 Phone (609) 258-5870
locationMoffett Laboratory
 
Faculty Assistant
Anna Schmedel
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Phone (609) 258-5028

 

Research Focus

Small-molecule-mediated interactions in complex microbial communities

Much like human beings, microbes often live in diverse communities interacting with both collaborators and competitors. Small molecule natural products mediate a significant portion of these interactions. As expected, the more complex a microbial community is, the richer its small molecule chemical arsenal becomes. This phenomenon has been observed in the complex microbiomes of marine invertebrates, terrestrial soils, human gut, and the plant rhizosphere, among others. Our research interests are mainly to study the chemical and biological interactions within complex microbial communities (microbe-microbe interactions) and between microbial communities and their multicellular hosts (microbe-host interactions). In respect to the human body and its microbial inhabitants (the human microbiome), these interactions can define the difference between commensals and pathogens, and thus between health and disease states. In the case of marine invertebrates (e.g., sponges and ascidians) and their symbionts, these interactions can provide the host with indispensible means of chemical defense, allowing it to survive in a predator-rich environment. In the soil or seawater, these interactions can dictate the microbial community’s composition, fitness and stability over time.  Our ongoing efforts towards understanding these interactions will not only explain fundamentals of basic biology in these systems, but will also supply a suite of biologically active small molecules that can be developed as therapeutic agents.

In addition, the Donia lab has a special interest in the uncultivable microbial components of complex communities, which have eluded research attempts for decades despite their abundance and clear importance. Recent advances in the fields of metagenomics and single-cell genomics have allowed access to the genetic information of some of these unculturable microbes, while functional studies remain challenging. Our goal is to develop the necessary computational and experimental tools to functionally study the interactions mediated by uncultivable members of complex microbiomes, using an integrated multi “omics” approach, including metagenomics, metabolomics and metatranscriptomics. The Donia lab functions at the intersection between multiple disciplines: microbiology, molecular biology, biochemistry, small molecule chemistry and biosynthesis, metagenomics and bioinformatics, aiming to answer basic biological questions and to develop new therapeutics.


Selected Publications

Donia MS, Cimermancic P, Schulze CJ,...Fischbach MA. (2014) A systematic analysis of biosynthetic gene clusters in the human microbiome reveals a common family of antibiotics. Cell. 158: 1402-1414. Highlighted in Nature, Nature Reviews Microbiology, The New York Times, The Economist, The Huffington Post, Science News, Bioworld, and Biotechniques. PubMed

Williams BB, Van Benschoten AH, Cimermancic P, Donia MS,...Fischbach MA. (2014) Discovery and characterization of gut microbiota decarboxylases that can produce the neurotransmitter tryptamine. Cell Host & Microbe. 16: 1-9.  PubMed

Kwan JC, Tianero MD, Donia MS, Wyche TP, Bugni, TS, Schmidt EW. (2014) Host control of symbiont natural product chemistry in cryptic populations of the tunicate Lissoclinum patella. PLoS One. 5: e95850 PubMed

Marcobal A, Kashyap PC, Nelson TA,...Donia MS,...Sonnenburg JL. (2013) A metabolomic view of how the human gut microbiota impacts the host metabolome using humanized and gnotobiotic mice. ISME J.  PubMed

Donia MS, Fischbach MA. (2013) Dyeing to learn more about the gut microbiota. Cell Host Microbe. 13: 119-120. PubMed

Kwan JC,; Donia MS, Han AW, Hirose E, Haygood MG, Schmidt EW. (2012) Genome streamlining and chemical defense in a coral reef symbiosis. Proc. Natl. Acad. Sci. 109: E20655-60 PubMed

Schmidt EW, Donia MS, McIntosh JA, Fricke WF, Ravel J. (2012) Origin and variation of tunicate secondary metabolites. J. Nat. Prod. 75: 295-304 PubMed

Tianero Ma D, Donia MS, Young TS, Schultz PG, Schmidt EW. (2012) Ribosomal route to small molecule diversity. J. Am. Chem. Soc. 134: 418-425 PubMed

Donia MS, Fricke WF, Partensky F,...Schmidt, EW. (2011) Complex microbiome underlying secondary and primary metabolism in the tunicate-Prochloron symbiosis. Proc. Natl. Acad. Sci. 108: E1423-1432 PubMed

Highlighted in Nature Biotechnology (2012) 30: 1.

McIntosh JA, Donia MS, Schmidt, EW. (2011) Enzymatic basis of ribosomal peptide prenylation in cyanobacteria. J. Am. Chem. Soc. 133: 34, 13698-705. PubMed

Highlighted in Chem. & Eng. News 89: 32, 2011

Donia MS, Ruffner DE, Cao S, Schmidt EW. (2011) Accessing the hidden majority of marine natural products through metagenomics. Chembiochem. 12: 1230-6. PubMed

Donia MS, Fricke WF, Ravel, Schmidt EW. (2011) Variation in tropical reef symbiont metagenomes defined by secondary metabolism. PLoS One. 6: e17897 PubMed

Donia MS, Schmidt EW. (2011) Linking chemistry and genetics in the growing cyanobactin natural products family. Chem. & Biol. 18: 508-19. PubMed

Schmidt EW, Donia MS. (2010) Life in cellulose houses: symbiotic bacterial biosynthesis of ascidian drugs and drug leads. Curr. Opin. Biotechnol. 21: 827-833. PubMed

McIntosh JA, Donia MS, Schmidt EW. (2010) Insights into heterocyclization from two highly similar enzymes. J. Am. Chem. Soc. 132: 4089-91. PubMed

Donia MS, Schmidt EW. (2010) Cyanobactins – ubiquitous cyanobacterial ribosomal peptide metabolites. in Comprehensive Natural Products II Chemistry and Biology, Vol. 2 539-558 (Elsevier: Oxford).

Schmidt EW, Donia MS. (2009) Cyanobactin ribosomally synthesized peptides — a case of deep genome mining. Methods Enzymol. 458: 575-596. PubMed

McIntosh JA, Donia MS, Schmidt EW. (2009) Ribosomal peptide natural products: bridging the ribosomal and nonribosomal worlds. Nat. Prod. Rep. 26: 537-59. PubMed

Donia MS, Ravel J, Schmidt EW. (2008) A global assembly line for cyanobactins. Nature Chem. Biol. 4: 341-3. PubMed

Donia MS, Hathaway BJ, Sudek S, Haygood MG, Rosovitz MJ, Ravel J, Schmidt EW. (2006) Natural combinatorial peptide libraries in cyanobacterial symbionts of marine ascidians. Nature Chem. Biol. 2: 729-735. PubMed

Highlighted by Joern Piel in Nature Chem. Biol. 2006, 2, 12, 661-662.

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Lewis Thomas Laboratory at Princeton University

119 Lewis Thomas Laboratory
Washington Road, Princeton, NJ  08544-1014

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Website:  molbio.princeton.edu