Faculty & Research
banner_research_labs

Molecular Biology Faculty

John Storey

Professor of Molecular Biology
and the Lewis-Sigler Institute for Integrative Genomics

John Storey

This email address is being protected from spambots. You need JavaScript enabled to view it.
lab Storey Research Lab
Phone (609) 258-7014
locationCarl Icahn Lab
Phone Lab (609) 258-1331
Faculty Assistant
Lee Morgan
This email address is being protected from spambots. You need JavaScript enabled to view it.
Phone (609) 258-0859

Research Focus

My lab develops and applies quantitative methods in genomics. We are particularly focused on functional genomics problems involving high-dimensional data sets, such as that obtained from large-scale genotyping, gene expression monitoring, and mass spectroscopy based proteomics. Because our research deals with large amounts of noisy data, we also develop theory and methods for statistics and machine learning.

This is an especially exciting time for quantitative genomics, as many studies are underway that involve multiple types of large-scale data. For example, we are working on studies involving high-throughput measurements on mRNA expression, protein expression, metabolite levels, protein-DNA binding, chromatin structure, and DNA sequences.

The over-arching goal of our research is to utilize multiple sources of high-throughput genomic data to understand biological regulatory networks and the molecular basis of complex traits. This involves characterizing the "wiring diagram" of the molecular biology of the cell. The ultimate goal is to build a quantitative system for understanding how the hard-wired components of a cell, such as DNA sequence and epigenetic factors, interact with the environment to determine the dynamic molecular behavior of the cell, as manifested in variables such as RNA expression, protein expression, enzymatic activity, and eventually as complex traits.

Specific problems we are working on include:

  • Inferring causal regulatory networks from studies involving high-throughput molecular profiling (e.g., RNA and protein expression) and large-scale genotyping.
  • Decomposing sources of gene expression variation in complex clinical and experimental settings.
  • Understanding the genetic and epigenetic determinants of the gene expression program.
  • Developing quantitative approaches to providing a causal "molecular dissection" of complex traits.
  • Understanding the relationship between evolutionary forces driving natural genetic variation and its effect on variation in expression levels of gene products.
  • Developing new theory and methods for high-dimensional statistical inference, large-scale significance testing, and machine learning

Selected Publications

Robinson DG, Chen W, Storey JD, Gresham D. (2013) Design and analysis of bar-seq experiments. G3 (Bethesda). 4: 11-8. Pubmed

Chung NC, Storey JD. (2013) Statistical significance of variables driving systematic variation. arXiv:1308.6013 [stat.ME]

Jaffe AE, Storey JD, Ji H, Leek JT. (2013) Gene set bagging for estimating replicability of gene set analyses. BMC Bioinformatics. 14: 360. Pubmed

Marstrand TT and Storey JD. (2012) Identifying and mapping cell-type specific chromatin programming of gene expression. arXiv:1210.3313 [q-bio.QM]

Desai KH and Storey JD. (2012) Cross-dimensional inference of dependent high-dimensional data. J Amer Stat Assoc. 107: 135-151. DOI:10.1080/01621459.2011.645777

Leek JT, Johnson WE, Parker HS, Jaffe AE, Storey JD. (2012) The sva package for removing batch effects and other unwanted variation in high-throughput experiments. Bioinformatics. 28: 882-3. Pubmed

Xiao W, Mindrinos MN, Seok J, Cuschieri J, Cuenca AG [...] Inflammation and Host Response to Injury Large-Scale Collaborative Research Program. (2011) A genomic storm in critically injured humans. J Exp Med. 208: 2581-90. Pubmed

Desai KH, Tan CS, Leek JT, Maier RV, Tompkins RG, Storey JD; Inflammation and the Host Response to Injury Large-Scale Collaborative Research Program. (2011) Dissecting inflammatory complications in critically injured patients by within-patient gene expression changes: a longitudinal clinical genomics study. PLoS Med. 8: e1001093. Pubmed

Kanodia JS, Kim Y, Tomer R, Khan Z, Chung K, Storey JD, Lu H, Keller PJ, Shvartsman SY. (2011) A computational statistics approach for estimating the spatial range of morphogen gradients. Development. 138: 4867-74. Pubmed

Xu W, Seok J, Mindrinos MN, Schweitzer AC, Jiang H [...] Inflammation and Host Response to Injury Large-Scale Collaborative Research Program. (2011) Human transcriptome array for high-throughput clinical studies. Proc Natl Acad Sci 108: 3707-12. Pubmed

Woo S, Leek JT, Storey JD. (2011) A computationally efficient modular optimal discovery procedure. Bioinformatics. 27: 509-15. Pubmed

Mecham BH, Nelson PS, Storey JD. (2010) Supervised normalization of microarrays. Bioinformatics. 26: 1308-15. Pubmed

Kruglyak L, Storey JD. (2009) Cause and express. Nat Biotechnol. 27: 544-545. PubMed

Chen LS, Storey JD. (2008) Eigen-R2 for dissecting variation in high-dimensional studies. Bioinformatics 24: 2260-2262. PubMed

Käll L, Storey JD, MacCoss MJ, Noble WS. (2008) Assigning significance to peptides identified by tandem mass spectrometry using decoy databases. J Prot Res 7: 29-34. PubMed

Chen LS, Emmert-Streib F, Storey JD. (2007) Harnessing naturally randomized transcription to infer regulatory relationships among genes. Genome Biol 8: R219. PubMed

Leek JT, Storey JD. (2007) Capturing heterogeneity in gene expression studies by surrogate variable analysis. PLoS Genet 3: 1724-1735. PubMed

Akey JM, Biswas S, Leek JT, Storey JD. (2007) On the design and analysis of expression studies in human populations. Nature Genet 39: 807-808. PubMed

Storey JD, Dai JY, Leek JT. (2007) The optimal discovery procedure for large-scale significance testing, with applications to comparative microarray experiments. Biostatistics 8: 414-432. PubMed

Storey JD, Xiao W, Leek JT, Tompkins RG, Davis RW. (2005) Significance analysis of time course microarray experiments. Proc Natl Acad Sci 102: 12837-12842.PubMed

Brem RB*, Storey JD*, Whittle J, Kruglyak L. (2005) Genetic interactions between polymorphisms that affect gene expression in yeast. Nature 436: 701-703. *Joint first authors. PubMed

Storey JD, Akey JM, Kruglyak L. (2005) Multiple locus linkage analysis of genome-wide expression in yeast. PLoS Biol 3: 1380-1390. PubMed

Storey JD. (2003) The positive false discovery rate: A Bayesian interpretation and the q-value. Ann Stat 31: 2013-2035.Storey JD, Tibshirani R. (2003) Statistical significance for genome-wide studies. Proc Natl Acad Sci 100: 9440-9445. PubMed

Arava Y, Wang Y, Storey JD, Brown PO, Herschlag D. (2003) Genome-wide analysis of mRNA translation profiles in Saccharomyces cerevisiae. Proc Natl Acad Sci 100: 3889-3894. PubMed

Wang Y, Liu CL, Storey JD, Tibshirani RJ, Herschlag D, Brown PO. (2002) Precision and functional specificity in mRNA decay. Proc Natl Acad Sci 99: 5860-5865. PubMed

Efron B, Tibshirani R, Storey JD, Tusher V. (2001) Empirical Bayes analysis of a microarray experiment. J Am Stat Assoc 96: 1151-1160. Storey JD, Siegmund D. (2001) Approximate p-values for local sequence alignments: numerical studies. J Comp Biol 8: 549-556. PubMed

MolBio Events

Wed, Apr 16, 2014

Thu, Apr 17, 2014

Fri, Apr 18, 2014

Mon, Apr 21, 2014

Contact Us

Lewis Thomas Laboratory at Princeton University

119 Lewis Thomas Laboratory
Washington Road, Princeton, NJ  08544-1014

Tel: (609) 258-3658
Fax: (609) 258-3980
Website:  molbio.princeton.edu