Molecular Biology Faculty
Jeffry B. Stock
Lewis Thomas Lab, 148
Lab (609) 258-6112
We are primarily concerned with the development of novel pharmaceuticals to regulate signal transduction pathways that underlie chronic diseases associated with inflammation and aging. The activities of approximately a third of the proteins in vertebrate cells are regulated by reversible phosphorylation at serine and threonine resides. There are almost a thousand different protein kinases, each of which catalyzes the transfer of phosphoryl groups from ATP to a specific set of protein substrates in response to a regulatory input. In contrast, there are just a few phosphoprotein phosphatases to catalyze the removal of these groups. Our research over the past several years has focused on the molecular mechanisms that underlie the regulation of these phosphatases. Through these efforts we have identified potential pharmaceutical targets for the global regulation of phosphatase activity, developed screens for useful therapeutic agents, and characterized pharmaceutical leads that provide a basis for further medicinal chemistry exploration. Our current research is essentially translational, with two principal targets: skin and brain.
The skin project targets inflammatory diseases such as psoriasis, rosacea, atopic dermatitis, and acne as well as the general problem of skin aging. Our brain research is concerned with neurodegenerative diseases such as Parkinson’s and Alzheimer’s. Both projects focus on the regulation and pharmacuetical modulation of the most abundant phosphoprotein phosphatase, phosphoprotein phosphatase 2A (PP2A). Several years ago we discovered that PP2A activity is regulated by reversible carboxyl methylation. This modification is catalyzed by two PP2A-specific converter enzymes, a methyltransferase (PPMT) and a methylesterase (PME). Demethylation is associated with inflammation, apoptosis, and neurodegeneration. We have identified several different pharmaceutical chemotypes that bind to PP2A and inhibit its demethylation by PME. We have shown that one agent, a natural product found in coffee, is efficacious in animal models for neurodegeneration and skin inflammation.
Xing Y, Li Z, Chen Y, Stock JB, Jeffrey PD, Shi Y. (2008) Structural mechanism of demethylation and inactivation of protein phosphatase 2A. Cell 133: 154-163. PubMed
Baker MD, Stock JB. (2007) Signal transduction: networks and integrated circuits in bacterial cognition. Curr Biol 17: R1021-1024. PubMed
Chen Y, McQuade KJ, Guan XJ, Thomason PA, Wert MS, Stock JB, Cox EC. (2007) Isoprenylcytseine carboxyl methylation is essential for development in Dictyostelium discoideum. Mol Biol Cell 18: 4106-1418. PubMed
Chen Y, Xu Y, Bao Q, Xing Y, Li Z, Lin Z, Stock JB, Jeffrey PD, Shi Y (2007). Structural and biochemical insights into the regulation of protein phosphatase 2A by small t antigen of SV40. Nat Struct Mol Biol 6: 527-534. PubMed
Xing Y, Xu Y, Chen Y, Jeffrey PD, Chao Y, Lin Z, Li Z, Strack S, Stock JB, Shi Y (2006). Structure of protein phosphatase 2A core enzyme bound to tumor-inducing toxins. Cell 127: 341-353. PubMed
Chao Y, Xing Y, Chen Y, Xu Y, Lin Z, Li Z, Jeffrey PD, Stock JB, Shi Y (2006). Structure and mechanism of the phosphotyrosyl phosphatase activator. Mol Cell 23: 535-546. PubMed
Wolanin PM, Baker MD, Francis NR, Thomas DR, Derosier DJ, Stock JB (2006). Self-assembly of receptor/signaling complexes in bacterial chemotaxis. Proc Natl Acad Sci USA 103: 14313-14318. PubMed
Thomason PA, Sawai S, Stock JB, Cox EC (2006). The histidine kinase homologue DhkK/Sombrero controls morphogenesis in Dictyostelium. Dev Biol 292: 358-370. PubMed
Baker M, Wolanin P and Stock J (2005). Systems biology of bacterial chemotaxis. Curr Opin Microbiol 9: 187-192. PubMed
Baker M, Wolanin P and Stock J (2005). Signal transduction in bacterial chemotaxis. Bioessays 28: 9-22. PubMed
Wolanin PM and Stock JB (2004). Bacterial chemosensing: cooperative molecular logic. Curr Biol 14: R486-487. PubMed
Webre DJ, Wolanin PM and Stock JB (2004). Modulated receptor interactions in bacterial transmembrane signaling. Trends Cell Biol 14: 478-482. PubMed
Lalchandani S and Stock J (2004). Protein Carboxyl Methylation: An Overview. In: Encyclopedia of Biological Chemistry, Academic Press 3: 474.
Francis NR, Wolanin PM, Stock JB, Derosier DJ and Thomas DR (2004). Three-dimensional structure and organization of a receptor/signaling complex. Proc Natl Acad Sci USA 101: 17480-17485. PubMed
Wolanin PM, Webre DJ and Stock JB (2003). Mechanism of phosphatase activity in the chemotaxis response regulator CheY. Biochemistry 42: 14075-14082. PubMed
Webre DJ, Wolanin PM and Stock JB (2003). Bacterial chemotaxis. Curr Biol 13: R47-49. PubMed
Park S, Wolanin PM, Yuzbashyan EA, Silberzan P, Stock JB, Austin RH (2003). Motion to form a quorum. Science 301: 188. PubMed
Park S, Wolanin PM, Yuzbashyan EA, Lin H, Darnton NC, Stock JB, Silberzan P, Austin R (2003). Influence of topology on bacterial social interaction. Proc Natl Acad Sci USA 100: 13910-13915. PubMed
Napper S, Wolanin PM, Webre DJ, Kindrachuk J, Waygood B, Stock JB (2003). Intramolecular rearrangements as a consequence of the dephosphorylation of phosphoaspartate residues in proteins. FEBS Lett 538: 77-80. PubMed
Wolanin PM, Thomason PA and Stock JB (2002). Histidine protein kinases: key signal transducers outside the animal kingdom. Genome Biol 3: REVIEWS3013. PubMed
Wolanin P and Stock J (2002). Transmembrane signaling and the regulation of histidine kinase activity. In: Histidine kinases in signal transduction. Inouye M and Dutta R, eds. New York, NY, Academic Press: 73-122.
Vafai SB and Stock JB (2002). Protein phosphatase 2A methylation: a link between elevated plasma homocysteine and Alzheimer's Disease. FEBS Lett 518: 1-4. PubMed
Thomason PA, Wolanin PM and Stock JB (2002). Signal transduction: receptor clusters as information processing arrays. Curr Biol 12: R399-401. PubMed
Stock JB, Levit MN and Wolanin PM (2002). Information processing in bacterial chemotaxis. Sci STKE 2002: PE25. PubMed
Levit MN and Stock JB (2002). Receptor methylation controls the magnitude of stimulus-response coupling in bacterial chemotaxis. J Biol Chem 277: 36760-36765. PubMed
Levit MN, Grebe TW and Stock JB (2002). Organization of the receptor-kinase signaling array that regulates Escherichia coli chemotaxis. J Biol Chem 277: 36748-36754. PubMed
Levit MN, Abramczyk BM, Stock JB and Postel EH (2002). Interactions between Escherichia coli nucleoside-diphosphate kinase and DNA. J Biol Chem 277: 5163-5167. PubMed
Francis NR, Levit MN, Shaikh TR, Melanson LA, Stock JB, DeRosier DJ (2002). Subunit organization in a soluble complex of tar, CheW, and CheA by electron microscopy. J Biol Chem 277: 36755-36759. PubMed
Da Re S, Tolstykh T, Wolanin PM and Stock JB (2002). Genetic analysis of response regulator activation in bacterial chemotaxis suggests an intermolecular mechanism. Protein Sci 11: 2644-2654. PubMed
Mourey L, Da Re S, Pedelacq JD, Tolstykh T, Faurie C, Guillet V, Stock JB, Samama JP (2001). Crystal structure of the CheA histidine phosphotransfer domain that mediates response regulator phosphorylation in bacterial chemotaxis. J Biol Chem 276: 31074-31082. PubMed
Wu J, Tolstykh T, Lee J, Boyd K, Stock JB, Broach JR (2000). Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo. EMBO J 19: 5672-5681. PubMed
Tolstykh T, Lee J, Vafai S and Stock JB (2000). Carboxyl methylation regulates phosphoprotein phosphatase 2A by controlling the association of regulatory B subunits. EMBO J 19: 5682-5691. PubMed
Stock JB and Da Re S (2000). Chemotaxis. In: Encyclopedia of Microbiology. Lederberg J, ed. San Diego, CA, Academic Press: 772-780.
Stock J and Levit M (2000). Signal transduction: hair brains in bacterial chemotaxis. Curr Biol 10: R11-14. PubMed
Stock J and Da Re S (2000). Signal transduction: response regulators on and off. Curr Biol 10: R420-424. PubMed
Thomason PA, Traynor D, Stock JB and Kay RR (1999). The RdeA-RegA system, a eukaryotic phospho-relay controlling cAMP breakdown. J Biol Chem 274: 27379-27384. PubMed
Stock J and Da Re S (1999). A receptor scaffold mediates stimulus-response coupling in bacterial chemotaxis. Cell Calcium 26: 157-164. PubMed
Stock J (1999). Sensitivity, cooperativity and gain in chemotaxis signal transduction. Trends Microbiol 7: 1-4. PubMed
Stock J (1999). Signal transduction: Gyrating protein kinases. Curr Biol 9: R364-367. PubMed
Levit MN and Stock JB (1999). pH sensing in bacterial chemotaxis. Novartis Found Symp 221: 38-50; discussions 50-54. PubMed
Levit MN, Liu Y and Stock JB (1999). Mechanism of CheA protein kinase activation in receptor signaling complexes. Biochemistry 38: 6651-6658. PubMed
Hakenbeck R, Grebe T, Zahner D and Stock JB (1999). beta-lactam resistance in Streptococcus pneumoniae: penicillin-binding proteins and non-penicillin-binding proteins. Mol Microbiol 33: 673-678. PubMed
Grebe TW and Stock JB (1999). The histidine protein kinase superfamily. Adv Microb Physiol 41: 139-227. PubMed
Elowitz MB, Surette MG, Wolf PE, Stock JB and Leibler S (1999). Protein mobility in the cytoplasm of Escherichia coli. J Bacteriol 181: 197-203. PubMed
Djordjevic S, Stock AM, Chen Y and Stock JB (1999). Protein methyltransferases involved in signal transduction. S-Adenosylmethionine-Dependent Methyltransferases: Structures and Functions. Cheng X and Blumenthal RM, eds. Singapore, World Scientific Publishing Co: 149-184.
Da Re SS, Deville-Bonne D, Tolstykh T, M Vr and Stock JB (1999). Kinetics of CheY phosphorylation by small molecule phosphodonors. FEBS Lett 457: 323-326. PubMed
Levit MN, Liu Y and Stock JB (1998). Stimulus response coupling in bacterial chemotaxis: receptor dimers in signalling arrays. Mol Microbiol 30: 459-466. PubMed
Grebe TW and Stock J (1998). Bacterial chemotaxis: the five sensors of a bacterium. Curr Biol 8: R154-157. PubMed
Alon U, Camarena L, Surette MG, Aguera y Arcas B, Liu Y, Leibler S, Stock J (1998). Response regulator output in bacterial chemotaxis. EMBO J 17: 4238-4248. PubMed
Liu Y, Levit M, Lurz R, Surette MG and Stock JB (1997). Receptor-mediated protein kinase activation and the mechanism of transmembrane signaling in bacterial chemotaxis. EMBO J 16: 7231-7240. PubMed
Elowitz MB, Surette MG, Wolf PE, Stock J and Leibler S (1997). Photoactivation turns green fluorescent protein red. Curr Biol 7: 809-812. PubMed
Zahner D, Grebe T, Guenzi E, Krauss J, van der Linden M, Terhune K, Stock JB, Hakenbeck R (1996). Resistance determinants for beta-lactam antibiotics in laboratory mutants of Streptococcus pneumoniae that are involved in genetic competence. Microb Drug Resist 2: 187-191. PubMed
Surette MG and Stock JB (1996). Role of alpha-helical coiled-coil interactions in receptor dimerization, signaling, and adaptation during bacterial chemotaxis. J Biol Chem 271: 17966-17973. PubMed
Surette MG, Levit M, Liu Y, Lukat G, Ninfa EG, Ninfa A, Stock JB (1996). Dimerization is required for the activity of the protein histidine kinase CheA that mediates signal transduction in bacterial chemotaxis. J Biol Chem 271: 939-945. PubMed
Stock J and Surette MG (1996). Chemotaxis. In: Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology. Ingraham JL, ed. Washington DC, American Society of Microbiology: 1103-1129.
Stock J (1996). Receptor signaling: dimerization and beyond. Curr Biol 6: 825-827. PubMed
Levit M, Liu Y, Surette M and Stock J (1996). Active site interference and asymmetric activation in the chemotaxis protein histidine kinase CheA. J Biol Chem 271: 32057-32063. PubMed
Lee J, Chen Y, Tolstykh T and Stock J (1996). A specific protein carboxyl methylesterase that demethylates phosphoprotein phosphatase 2A in bovine brain. Proc Natl Acad Sci USA 93: 6043-6047. PubMed
Hakenbeck R and Stock JB (1996). Analysis of two-component signal transduction systems involved in transcriptional regulation. Methods Enzymol 273: 281-300. PubMed
de Jonge R, Teixeira de Mattos MJ, Stock JB and Neijssel OM (1996). Pyrroloquinoline quinone, a chemotactic attractant for Escherichia coli. J Bacteriol 178: 1224-1226. PubMed
Volker C and Stock JB (1995). Carboxyl methylation of Ras-related proteins. Methods Enzymol 255: 65-82. PubMed
Volker C, Pillinger MH, Philips MR and Stock JB (1995). Prenylcysteine analogs to study function of carboxylmethylation in signal transduction. Methods Enzymol 250: 216-225. PubMed
Stock J, Surette MG, Levit M and Park P (1995). Two-component signal transduction systems: structure-function relationships and mechanisms of catalysis. In: Two-Component Signal Transduction. Hoch JA and Silhavy TJ, eds. Washington DC, American Society of Microbiology: 25-51.
Regazzi R, Sasaki T, Takahashi K, Jonas JC, Volker C, Stock JB, Takai Y, Wollheim CB (1995). Prenylcysteine analogs mimicking the C-terminus of GTP-binding proteins stimulate exocytosis from permeabilized HIT-T15 cells: comparison with the effect of Rab3AL peptide. Biochim Biophys Acta 1268: 269-278. PubMed
Philips MR, Staud R, Pillinger M, Feoktistov A, Volker C, Stock JB, Weissmann G (1995). Activation-dependent carboxyl methylation of neutrophil G-protein gamma subunit. Proc Natl Acad Sci USA 92: 2283-2287. PubMed
Surette MG and Stock JB (1994). Transmembrane signal transducing proteins. In: New Comprehensive Biochemistry. Ghuysen JM and Hakenbeck R, eds. Amsterdam, Elsevier Science B.V. Vol. 27. Bacterial Cell Wall: 465-483.
Surette MG and Stock JB (1994). Enzymatic protein methylation, demethylation, and deamidation: Its role in sensory transduction in chemotactic bacteria. Deamidation and Isoaspartate Formation in Peptides and Proteins. Aswad DW, ed. Boca Raton, FL, CRC Press, Inc: 207-228.
Stock JB (1994). Adaptive responses in bacterial chemotaxis. In: Regulation of Cellular Signal Transduction Pathways by Desensitization and Amplification. Sibley D and Houslay M, eds. Baffins Lane, Chichester, John Wiley and Sons, Ltd: 3-24.
Stock J, Surette M and Park P (1994). Chemosensing and signal transduction in bacteria. Curr Opin Neurobiol 4: 474-480. PubMed
Stock J and Surette M (1994). Bacterial chemotaxis. The motor connection. Curr Biol 4: 143-144. PubMed
Pillinger MH, Volker C, Stock JB, Weissmann G and Philips MR (1994). Characterization of a plasma membrane-associated prenylcysteine-directed alpha carboxyl methyltransferase in human neutrophils. J Biol Chem 269: 1486-1492. PubMed
McCleary WR and Stock JB (1994). Acetyl phosphate and the activation of two-component response regulators. J Biol Chem 269: 31567-31572. PubMed
Floer M and Stock J (1994). Carboxyl methylation of protein phosphatase 2A from Xenopus eggs is stimulated by cAMP and inhibited by okadaic acid. Biochem Biophys Res Commun 198: 372-379. PubMed
Stock J (1993). Protein phosphorylation: phosphoprotein talk. Curr Biol 3: 303-305. PubMed
Stock AM, Martinez-Hackert E, Rasmussen BF, West AH, Stock JB, Ringe D, Petsko GA (1993). Structure of the Mg(2+)-bound form of CheY and mechanism of phosphoryl transfer in bacterial chemotaxis. Biochemistry 32: 13375-13380. PubMed
Philips MR, Pillinger MH, Staud R, Volker C, Rosenfeld MG, Weissmann G, Stock JB (1993). Carboxyl methylation of Ras-related proteins during signal transduction in neutrophils. Science 259: 977-980. PubMed
Metz SA, Rabaglia ME, Stock JB and Kowluru A (1993). Modulation of insulin secretion from normal rat islets by inhibitors of the post-translational modifications of GTP-binding proteins. Biochem J 295 (Pt 1): 31-40. PubMed
McCleary WR, Stock JB and Ninfa AJ (1993). Is acetyl phosphate a global signal in Escherichia coli? J Bacteriol 175: 2793-2798. PubMed
McCleary WR and Stock JB (1993). Phosphorylation in bacterial chemotaxis. In: Signal Transduction: Prokaryotic and Simple Eukaryotic Systems. Kurjan J and Taylor BL eds. New York NY, Academic Press Inc: 17-41.
Lukat GS and Stock JB (1993). Response regulation in bacterial chemotaxis. J Cell Biochem 51: 41-46. PubMed
Lee J and Stock J (1993). Protein phosphatase 2A catalytic subunit is methyl-esterified at its carboxyl terminus by a novel methyltransferase. J Biol Chem 268: 19192-19195. PubMed
DeKoster GT, Robertson AD, Stock AM and Stock JB (1993). Urea and guanidine-HCl yield different unfolding free energies for CheY: Which denaturant provides the most reliable free energy values? Tech Prot Chem IV: 533-540.
Akbar H, Wang W, Kornhauser R, Volker C and Stock JB (1993). Protein prenylcysteine analog inhibits agonist-receptor-mediated signal transduction in human platelets. Proc Natl Acad Sci USA 90: 868-872.
Stock JB, Surette MG, McCleary WR and Stock AM (1992). Signal transduction in bacterial chemotaxis. J Biol Chem 267: 19753-19756. PubMed
Nathke IS, Heuser J, Lupas A, Stock J, Turck CW, Brodsky FM (1992). Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68: 899-910. PubMed
Lupas AN, Lupas JM and Stock JB (1992). Do G protein subunits associate via a three-stranded coiled coil? FEBS Lett 314: 105-108. PubMed
Lukat GS, McCleary WR, Stock AM and Stock JB (1992). Phosphorylation of bacterial response regulator proteins by low molecular weight phospho-donors. Proc Natl Acad Sci USA 89: 718-722. PubMed
Krueger JK, Stock J and Schutt CE (1992). Evidence that the methylesterase of bacterial chemotaxis may be a serine hydrolase. Biochim Biophys Acta 1119: 322-326. PubMed
Feng J, Atkinson MR, McCleary W, Stock JB, Wanner BL, Ninfa AJ (1992). Role of phosphorylated metabolic intermediates in the regulation of glutamine synthetase synthesis in Escherichia coli. J Bacteriol 174: 6061-6070. PubMed
Camps M, Hou C, Sidiropoulos D, Stock JB, Jakobs KH, Gierschik P (1992). Stimulation of phospholipase C by guanine-nucleotide-binding protein beta gamma subunits. Eur J Biochem 206: 821-831. PubMed
Volker C, Miller RA, McCleary WR, Rao A, Poenie M, Backer JM, Stock JB (1991). Effects of farnesylcysteine analogs on protein carboxyl methylation and signal transduction. J Biol Chem 266: 21515-21522. PubMed
Volker C, Lane P, Kwee C, Johnson M and Stock J (1991). A single activity carboxyl methylates both farnesyl and geranylgeranyl cysteine residues. FEBS Lett 295: 189-194. PubMed
Stock JB, Lukat GS and Stock AM (1991). Bacterial chemotaxis and the molecular logic of intracellular signal transduction networks. Annu Rev Biophys Biophys Chem 20: 109-136. PubMed
Stock J (1991). Balancing effector outputs. Curr Biol 1: 154-156. PubMed
Ninfa EG, Stock A, Mowbray S and Stock J (1991). Reconstitution of the bacterial chemotaxis signal transduction system from purified components. J Biol Chem 266: 9764-9770. PubMed
Lupas A, Van Dyke M and Stock J (1991). Predicting coiled coils from protein sequences. Science 252: 1162-1164. PubMed
Lukat GS, Lee BH, Mottonen JM, Stock AM and Stock JB (1991). Roles of the highly conserved aspartate and lysine residues in the response regulator of bacterial chemotaxis. J Biol Chem 266: 8348-8354. PubMed
Stock JB, Stock AM and Mottonen JM (1990). Signal transduction in bacteria. Nature 344: 395-400. PubMed
Stock JB (1990). Protein methylation in bacterial chemotaxis. In: Protein Methylation. Paik WK and Kim S. Boca Raton FL, CRC Press: 275-284.
Stock J (1990). A membrane receptor kinase that regulates development in Bacillus subtilis. Bioessays 12: 387-388. PubMed
Stimmel JB, Deschenes RJ, Volker C, Stock J and Clarke S (1990). Evidence for an S-farnesylcysteine methyl ester at the carboxyl terminus of the Saccharomyces cerevisiae RAS2 protein. Biochemistry 29: 9651-9659. PubMed
Olmedo G, Ninfa EG, Stock J and Youngman P (1990). Novel mutations that alter the regulation of sporulation in Bacillus subtilis. Evidence that phosphorylation of regulatory protein SpoOA controls the initiation of sporulation. J Mol Biol 215: 359-372. PubMed
Lukat GS, Stock AM and Stock JB (1990). Divalent metal ion binding to the CheY protein and its significance to phosphotransfer in bacterial chemotaxis. Biochemistry 29: 5436-5442. PubMed
Krueger J, Stock A, Schutt C and Stock JB (1990). Inclusion bodies from proteins produced at high levels in E. coli. In: Protein Folding: Deciphering the Second Half of the Genetic Code. Gierasch LM and King J, eds. Washington DC, AAAS: 1236-1242.
Stock JB, Ninfa AJ and Stock AM (1989). Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev 53: 450-490. PubMed
Stock AM, Mottonen JM, Stock JB and Schutt CE (1989). Three-dimensional structure of CheY, the response regulator of bacterial chemotaxis. Nature 337: 745-749. PubMed
Lupas A and Stock J (1989). Phosphorylation of an N-terminal regulatory domain activates the CheB methylesterase in bacterial chemotaxis. J Biol Chem 264: 17337-17342. PubMed
Krueger JK, Kulke MH, Schutt C and Stock JB (1989). Protein inclusion body formation and purification. BioPharm 2: 40-45.
Igo MM, Ninfa AJ, Stock JB and Silhavy TJ (1989). Phosphorylation and dephosphorylation of a bacterial transcriptional activator by a transmembrane receptor. Genes Dev 3: 1725-1734. PubMed
Deschenes RJ, Stimmel JB, Clarke S, Stock J and Broach JR (1989). RAS2 protein of Saccharomyces cerevisiae is methyl-esterified at its carboxyl terminus. J Biol Chem 264: 11865-11873. PubMed
Wylie D, Stock A, Wong CY and Stock J (1988). Sensory transduction in bacterial chemotaxis involves phosphotransfer between Che proteins. Biochem Biophys Res Commun 151: 891-896. PubMed
Stock JB and Simms S (1988). Methylation, demethylation, and deamidation at glutamate residues in membrane chemoreceptor proteins. In: Post-translational Modifications of Proteins and Ageing. Zappia V, ed. London, Plenum Publishing Comp: 201-212.
Stock AM, Wylie DC, Mottonen JM, Lupas AN, Ninfa EG, Ninfa AJ, Schutt CE, Stock JB (1988). Phospho-proteins involved in bacterial signal transduction. Cold Spring Harbor Symp Quant Biol 53: 49-57. PubMed
Stock A, Chen T, Welsh D and Stock J (1988). CheA protein, a central regulator of bacterial chemotaxis, belongs to a family of proteins that control gene expression in response to changing environmental conditions. Proc Natl Acad Sci USA 85: 1403-1407. PubMed
Ninfa AJ, Ninfa EG, Lupas AN, Stock A, Magasanik B, Stock J (1988). Crosstalk between bacterial chemotaxis signal transduction proteins and regulators of transcription of the Ntr regulon: evidence that nitrogen assimilation and chemotaxis are controlled by a common phosphotransfer mechanism. Proc Natl Acad Sci USA 85: 5492-5496. PubMed
Clarke S, Vogel JP, Deschenes RJ and Stock J (1988). Posttranslational modification of the Ha-ras oncogene protein: evidence for a third class of protein carboxyl methyltransferases. Proc Natl Acad Sci USA 85: 4643-4647. PubMed
Stock JB and Stock AM (1987). What is the role of receptor methylation in bacterial chemotaxis? Trends Biochem. Sci. 12: 371-375.
Stock J (1987). Mechanisms of receptor function and the molecular biology of information processing in bacteria. Bioessays 6: 199-203. PubMed
Stock AM and Stock JB (1987). Purification and characterization of the CheZ protein of bacterial chemotaxis. J Bacteriol 169: 3301-3311. PubMed
Stock A, Schaeffer E, Koshland DE, Jr. and Stock J (1987). A second type of protein methylation reaction in bacterial chemotaxis. J Biol Chem 262: 8011-8014. PubMed
Stock A, Mottonen J, Chen T and Stock J (1987). Identification of a possible nucleotide binding site in CheW, a protein required for sensory transduction in bacterial chemotaxis. J Biol Chem 262: 535-537. PubMed
Stock A, Clarke S, Clarke C and Stock J (1987). N-terminal methylation of proteins: structure, function and specificity. FEBS Lett 220: 8-14. PubMed
Simms SA, Stock AM and Stock JB (1987). Purification and characterization of the S-adenosylmethionine:glutamyl methyltransferase that modifies membrane chemoreceptor proteins in bacteria. J Biol Chem 262: 8537-8543. PubMed
Simms SA, Cornman EW, Mottonen J and Stock J (1987). Active site of the enzyme which demethylates receptors during bacterial chemotaxis. J Biol Chem 262: 29-31. PubMed
Borczuk A, Stock A and Stock J (1987). S-adenosylmethionine may not be essential for signal transduction during bacterial chemotaxis. J Bacteriol 169: 3295-3300. PubMed
Borczuk A, Staub A and Stock J (1986). Demethylation of bacterial chemoreceptors is inhibited by attractant stimuli in the complete absence of the regulatory domain of the demethylating enzyme. Biochem Biophys Res Commun 141: 918-923. PubMed
Stock J, Kersulis G and Koshland DE, Jr. (1985). Neither methylating nor demethylating enzymes are required for bacterial chemotaxis. Cell 42: 683-690. PubMed
Stock J, Borczuk A, Chiou F and Burchenal JE (1985). Compensatory mutations in receptor function: a reevaluation of the role of methylation in bacterial chemotaxis. Proc Natl Acad Sci USA 82: 8364-8368. PubMed
Stock A, Koshland DE, Jr. and Stock J (1985). Homologies between the Salmonella typhimurium CheY protein and proteins involved in the regulation of chemotaxis, membrane protein synthesis, and sporulation. Proc Natl Acad Sci USA 82: 7989-7993. PubMed
Simms SA, Keane MG and Stock J (1985). Multiple forms of the CheB methylesterase in bacterial chemosensing. J Biol Chem 260: 10161-10168. PubMed
Stock JB, Clarke S and Koshland DE, Jr. (1984). The protein carboxylmethyltransferase involved in Escherichia coli and Salmonella typhimurium chemotaxis. Methods Enzymol 106: 310-321. PubMed
Stock J and Koshland DE, Jr. (1984). Sensory adaptation mechanisms in swarm development. In: Microbial Development. Shapiro L and Losick R, eds. Cold Springs Harbor, New York: 117-131.
Snyder MA, Stock JB and Koshland DE, Jr. (1984). Carboxylmethyl esterase of bacterial chemotaxis. Methods Enzymol 106: 321-330. PubMed
Stock JB, Waygood EB, Meadow ND, Postma PW and Roseman S (1982). Sugar transport by the bacterial phosphotransferase system. The glucose receptors of the Salmonella typhimurium phosphotransferase system. J Biol Chem 257: 14543-14552. PubMed
Koshland DE, Jr., Goldbeter A and Stock JB (1982). Amplification and adaptation in regulatory and sensory systems. Science 217: 220-225. PubMed
Stock JB, Maderis AM and Koshland DE, Jr (1981). Bacterial chemotaxis in the absence of receptor carboxylmethylation. Cell 27: 37-44. PubMed
Stock JB and Koshland DE, Jr (1981). Changing reactivity of receptor carboxyl groups during bacterial sensing. J Biol Chem 256: 10826-10833. PubMed
Stock JB and Koshland DE, Jr (1981). A cyclic mechanism for excitation and adaptation. Curr Top Cell Regul 18: 505-517. PubMed
Snyder MA, Stock JB and Koshland DE, Jr (1981). Role of membrane potential and calcium in chemotactic sensing by bacteria. J Mol Biol 149: 241-257. PubMed
Postma PW and Stock JB (1980). Enzymes II of the phosphotransferase system do not catalyze sugar transport in the absence of phosphorylation. J Bacteriol 141: 476-484. PubMed
Stock JB and Koshland DE, Jr (1978). A protein methylesterase involved in bacterial sensing. Proc Natl Acad Sci USA 75: 3659-3663. PubMed
Stock JB and Koshland DE, Jr. (1978). Identification of a methyltransferase and a methylesterase with genes involved in bacterial chemotaxis. In: Transmethylation Usdin E, Borchardt RT and Crevekling CR, eds. New York, Elsevier: 595-602.
Stock JB, Rauch B and Roseman S (1977). Periplasmic space in Salmonella typhimurium and Escherichia coli. J Biol Chem 252: 7850-7861. PubMed
Stock J and Roseman S (1971). A sodium-dependent sugar co-transport system in bacteria. Biochem Biophys Res Commun 44: 132-138. PubMed