Princeton University Molecular Biology - Molecular Biology Events http://molbio.princeton.edu Sat, 13 Feb 2016 17:17:02 -0500 Joomla! - Open Source Content Management en-gb Inauguration of Nikon Center of Excellence at PU http://molbio.princeton.edu/events/event/450-inauguration-of-nikon-center-of-excellence-at-pu http://molbio.princeton.edu/events/event/450-inauguration-of-nikon-center-of-excellence-at-pu Location: Schultz Lab, 107 - Princeton
Category: Confocal Microscopy
Date: Tue, Jul 21, 2015 - Tue, Jul 21, 2015
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Grand Opening - Join us to celebrate the inauguration of the Nikon Center of Excellence at Princeton University.

Schedule of events
1:00 - 1:30pm Welcome/Introduction
1:30 - 2:30pm

"Understanding the Cell Biology of Collective Cell Behaviors"
Danelle Devenport, Ph.D., Assistant Professor, Department of Molecular Biology 

2:30 - 3:30pm

"Tuning in to the Right Station: Using Optogenetics to Dissect Information Processing in Cell Signaling Networks"
Jared Toettcher, Ph.D., Assistant Professor, Department of Molecular Biology 

3:30 - 4:30pm

Wine and Cheese Reception

 
Audience

Free and open to the university community and the public

About Nikon Center graphic

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Confocal Microscopy Mon, 18 Aug 2014 15:09:18 -0400
Molecular Biology Retreat 2015 http://molbio.princeton.edu/events/event/452-retreat-2015 http://molbio.princeton.edu/events/event/452-retreat-2015 Location: Frick Chemistry Laboratory - Princeton
Category: Retreat
Date: Fri, Oct 09, 2015 - Sat, Oct 10, 2015
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Invitation Only

October 9-10, 2015

DESCRIPTION

The retreat is a wonderful opportunity for our expansive and diverse department to engage scientifically, form collaborations, and interact in an informal setting. It is also a part of our graduate program, giving graduate students and post-docs the chance to present their research in the form of posters and talks.

Registration

TBD

Coordinators

Mohamed Abou Donia and Jared Toettcher
Co-chairs, Molecular Biology Retreat

Audience

Invitation Only. Graduate Students, Postdocs, Faculty and Staff

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Retreat Tue, 30 Jul 2013 14:25:56 -0400
Scott Waddell (Oxford) http://molbio.princeton.edu/events/event/464-waddell http://molbio.princeton.edu/events/event/464-waddell Location: Lewis Thomas Lab, 003 - Princeton
Category: Butler Seminar Series
Date: Wed, Dec 16, 2015 - Wed, Dec 16, 2015
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Butler Seminar Series

Speaker

Photo of Scott Waddell

Scott Waddell, Ph.D.

Professor of Neurobiology
Physiology, Anatomy and Genetics
Medical Science Division
University of Oxford 

Seminar Topic

Neural mechanisms of behavioral control in Drosophila

Learning permits animals to attach meaning and context to sensory stimuli. We are interested in how memories are coded in neural networks in the brain, and appropriately retrieved and utilized to guide behavior. In the fruit fly olfactory memories of particular value are represented within sparse populations of odor-activated Kenyon cells (KCs) in the mushroom body ensemble. Current models suggest that during learning reinforcing dopaminergic neurons skew the mushroom body network by driving zonally-restricted plasticity at synaptic junctions between the KCs and subsets of the overall small collection of mushroom body output neurons. Reactivation of the skewed KC-output neuron network retrieves memory of odor valence and guides appropriate approach or avoidance behavior. Our recent work suggests that skews in the KC-output neuron network might also account for state-dependent memory retrieval and memory re-evaluation.

Research LAB

Waddell Group

Audience

Free and open to the university community and the public

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Butler Seminar Series Mon, 18 Aug 2014 15:09:18 -0400
Ajeet Singh (Max Planck Institute for Developmental Biology) http://molbio.princeton.edu/events/event/491-singh http://molbio.princeton.edu/events/event/491-singh Location: Schultz Lab, 107 - Princeton
Category: Special Seminar
Date: Wed, Jan 06, 2016 - Wed, Jan 06, 2016
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Special Seminar

Speaker
Ajeet Singh
Max Planck Institute for Developmental Biology

Ajeet is currently a postdoctoral fellow in the Nüsslein-Volhard lab at the Max Planck Institute for Developmental Biology, Tuebingen. He is broadly interested in understanding how diverse cell types are generated from multipotent adult progenitors and how these cells interact to give rise to complex patterns over a tissue and organism-scale. 

Seminar Topic

Cellular Basis for Color Pattern in Zebrafish

Audience

Free and open to the university community and the public.

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Special Seminar Mon, 18 Aug 2014 15:09:18 -0400
Sarah A. Port (U of Göttingen) http://molbio.princeton.edu/events/event/494-port http://molbio.princeton.edu/events/event/494-port Location: Schultz Lab, 318 - Princeton
Category: Informal Seminar
Date: Mon, Jan 11, 2016 - Mon, Jan 11, 2016
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Informal Seminar

Speaker

Sarah A. Port, Ph.D.
Department of Molecular Biology
University of Göttingen 

Seminar Topic

"Structural and Functional Characterization of CRM1-Nup214 Interactions Reveals Multiple FG-Binding Sites Involved in Nuclear Export"

Audience

Free and open to the university community and the public.

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Informal Seminar Mon, 18 Aug 2014 15:09:18 -0400
Martin Jonikas (Carnegie Institution for Science) http://molbio.princeton.edu/events/event/489-jonikas http://molbio.princeton.edu/events/event/489-jonikas Location: Schultz Lab, 107 - Princeton
Category: Special Seminar
Date: Mon, Jan 11, 2016 - Mon, Jan 11, 2016
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Special Seminar

Speaker
Jonikas
Martin Jonikas
Carnegie Institution for Science
Martin Jonikas is a Young Investigator at the Carnegie Institution for Science and an Assistant Professor by courtesy at Stanford University. His laboratory aims to enable transformative advances in sustainability by bringing systems and synthetic biology to photosynthetic organisms. He studied aerospace engineering as an undergraduate at the Massachusetts Institute of Technology. He then received his Ph.D. from the University of California, San Francisco working with Jonathan Weissman, Maya Schuldiner and Peter Walter on high-throughput genetics and protein folding in the endoplasmic reticulum. He is the recipient of several awards, including a 2015 NIH New Innovator Award, a 2010 Air Force Young Investigator Award and a 2005 National Science Foundation Graduate Research Fellowship.
Seminar Topic

Systems and Synthetic Biology of Photosynthetic Organisms

Jonikas seminar illustration

Photosynthetic organisms, our planet's greatest chemists, form the foundations for human health by providing us with food, fuel, materials and drugs. Yet, our understanding of these organisms is in its infancy. My laboratory aims to enable transformative advances in sustainability by bringing systems and synthetic biology to photosynthetic organisms.

We have brought high-throughput genetics to photosynthetic eukaryotes by generating the first indexed, genome-wide collection of mutants in a single-celled photosynthetic organism, the green alga Chlamydomonas reinhardtii. We have also developed tools for systematically determining the localizations of hundreds of proteins in this organism.

I will illustrate how these tools have allowed us to discover the mechanism of formation of the pyrenoid, a mysterious and overlooked algal organelle that performs approximately one-third of global carbon fixation. I will further discuss our early efforts as part of an international collaboration to transfer this organelle into crops to enhance their productivity.

Research Lab

https://dpb.carnegiescience.edu/labs/jonikas-lab

Audience

Free and open to the university community and the public

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Special Seminar Mon, 18 Aug 2014 15:09:18 -0400
Chan Tu (Columbia U) http://molbio.princeton.edu/events/event/495-tu http://molbio.princeton.edu/events/event/495-tu Location: Schultz Lab, 107 - Princeton
Category: Special Seminar
Date: Wed, Jan 20, 2016 - Wed, Jan 20, 2016
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Special Seminar

Speaker
Chan Tu
Chan Thu
Columbia University
Department of Biochemistry and Molecular Biophysics 
Seminar Topic

Molecular mechanisms of neuronal self-recognition mediated by the clustered protocadherins

thu-scientificimageA key principle of neural circuit assembly is neurite self-avoidance, whereby sister axons or dendrites of the same neuron repel each other. This process is necessary to achieve efficient coverage of projection or receptive fields. Remarkably, however, sister neurites engaged is self-avoidance interact freely with other neurons, indicating that   neurons can discriminate “self” from “non-self”. Self-identity of neuron is determined by stochastic expression of a distinct set of cadherin superfamily members called clustered protocadherin (Pcdh) α, β and γ proteins in mammals.

In order to better understand to role of individual Pcdh isoforms in generating single cell diversity, we have carried out detailed functional and structural studies of the clustered Pcdhs (in collaboration with the groups of Larry Shapiro and Barry Honig at Columbia). Using a cell aggregation assay for homophilic interactions of distinct Pcdh isoforms, we have found that individual Pcdh α, β and γ protein isoforms exhibit strict homophilic binding, and that multiple distinct Pcdh isoforms can act combinatorially to generate unique multi- isoform trans binding specificities. Based on our findings from X-ray 3D structures and computational studies, we proposed that sister neurites from the same neuron can form a large assembly or lattice of Pcdh units through specific homophilic interactions. In contrast, non-sister neurites displaying different Pcdh isoform compositions would incorporate mismatches, preventing the formation of the lattice.  Thus, the size of Pcdh complexes or lattice at the site of contacts may determine the strength of downstream signaling leading to neurite repulsion in vivo. Our detailed structure studies provide the molecular nature of Pcdh homophilic interactions and the mechanisms by which the clustered Pcdhs provide individual neurons with a unique cell surface identity.

Audience

Free and open to the university community and the public

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Special Seminar Mon, 18 Aug 2014 15:09:18 -0400
Jeannie T. Lee (Harvard) http://molbio.princeton.edu/events/event/466-lee http://molbio.princeton.edu/events/event/466-lee Location: Lewis Thomas Lab, 003 - Princeton
Category: Butler Seminar Series
Date: Wed, Jan 27, 2016 - Wed, Jan 27, 2016
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Butler Seminar Series

Speaker

Jeannie

Jeannie T. Lee, M.D., Ph.D.
Professor of Genetics and Pathology
Harvard Medical School

Molecular Biologist
Massachusetts General Hospital
 
HHMI Investigator
Jeannie T. Lee is an Investigator of the Howard Hughes Medical Institute, a Professor of Genetics at Harvard Medical School, and a Member of the National Academy of Sciences. She is also Co-Director of the Harvard Epigenetics Initiative. Dr. Lee specializes in the study of epigenetic regulation by long noncoding RNAs and employs X-chromosome inactivation as a model system. For her work on RNA-mediated chromatin change, Dr. Lee became the recipient of the 2010 Molecular Biology Prize from the National Academy of Sciences, is a Fellow of the American Association for the Advancement of Science, and was named a Distinguished Graduate Award of the University of Pennsylvania School of Medicine. She received her A.B. in Biochemistry and Molecular Biology from Harvard University, obtained M.D.-Ph.D. degrees from the University of Pennsylvania School of Medicine, and did postgraduate training at the Whitehead Institute/MIT and at the Massachusetts General Hospital. She was also a Basil O’Connor Scholar and a Pew Scholars, and, until recently, served on the Board of Directors of the Genetics Society of America. In 2011, she co-founded RaNA Therapeutics to harness the potential of long noncoding RNAs to treat disease./td>
Seminar Topic

X-inactivation as a model for epigenetic regulation by long noncoding RNA

Research LAB

Jeannie Lee Lab

Audience

Free and open to the university community and the public

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Butler Seminar Series Mon, 18 Aug 2014 15:09:18 -0400
Shelby Blythe (Princeton U) http://molbio.princeton.edu/events/event/496-blythe http://molbio.princeton.edu/events/event/496-blythe Location: Lewis Thomas Lab, 003 - Princeton
Category: Special Seminar
Date: Wed, Feb 03, 2016 - Wed, Feb 03, 2016
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Special Seminar

Speaker
shelby
Shelby Blythe
Deapartment of Molecular Biology
Princeton University
Seminar Topic

Transcription, chromatin remodeling, and cell cycle control during early embryogenesis

Shelby ResearchHistorically, our knowledge of the genetic interactions that shape and pattern the embryo has far surpassed our understanding of how chromatin structure regulates gene expression. I have developed technologies for measuring chromatin accessibility (ATAC-seq) and occupancy (ChIP-seq) to address how changes in chromatin structure operate over short (3 to 20 minute) timescales to alter the gene-regulatory landscape in Drosophila embryos. With these approaches, I have measured the large scale remodeling events that establish a chromatin ‘ground state’, and have characterized how spatially restricted patterns of chromatin accessibility arise in response to patterning information. These large scale changes in chromatin structure are functionally linked to simultaneous cell cycle remodeling events. By exploring this functional interaction, I have developed genetic approaches for identifying rate-limiting factors for driving the large scale coordinated remodeling of embryonic promoters. These experiments serve as the basis for the future systematic examination of how fine-scale control of chromatin architecture underlies the embryonic developmental program.

Audience

Free and open to the university community and the public.

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Special Seminar Mon, 18 Aug 2014 15:09:18 -0400
SMART Goals for a Heart Healthy Lifestyle http://molbio.princeton.edu/events/event/497-smart http://molbio.princeton.edu/events/event/497-smart Location: Lewis Thomas Lab, 118 - Princeton
Category: Fun Committee
Date: Fri, Feb 05, 2016 - Fri, Feb 05, 2016
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Fun Committee Events

In honor of National Heart Month and Go Red for Women Day, University Health Coaches will offer a presentation for the MOL BIO community focused on providing easy-to-incorporate strategies to improve and maintain your heart health.  Learn to set SMART Goals for successful heart-healthy lifestyle.

resented by:  Gerry Pierre and Lisa Calabrese, University Health Coaches

Lunch will be available; attendance for this event is limited to 25.  RSVP by February 1.

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Fun Committee Mon, 18 Aug 2014 15:09:18 -0400
Yukiko Yamashita (U of Michigan) http://molbio.princeton.edu/events/event/465-yamashita http://molbio.princeton.edu/events/event/465-yamashita Location: Lewis Thomas Lab, 003 - Princeton
Category: Butler Seminar Series
Date: Wed, Feb 17, 2016 - Wed, Feb 17, 2016
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Butler Seminar Series

Speaker

Photo of Yukiko Yamashita

Yukiko Yamashita, Ph.D.
Associate Professor of Cell and Developmental Biology
University of Michigan

Research Associate Professor
Center for Stem Cell Biology
Life Sciences Institute
 
HHMI Investigator

Yukiko Yamashita obtained her Ph.D. from Kyoto University, Japan; completing the postdoctoral fellowship with Minx Fuller at Stanford University.  She started her own laboratory at the University of Michigan in 2007, and is currently an associate professor at the University of Michigan.

She studies how adult stem cells divide asymmetrically, giving rise to one stem cell and one differentiating cell, to maintain tissue homeostasis in the context of the stem cell niche. The particular focus is on how general cell biological processes, such as cell cycle regulation and cytoskeleton organization, which are shared by many other non-stem cells, are modulated in a stem cell-specific manner to fulfill stem cell function. 

Yamashita is a recipient of 2008 Searle Scholar Award, 2009 ASCB WICB junior award, 2011 MacArthur Fellowship, and is an HHMI investigator since 2014. 

Seminar Topic

Asymmetric stem cell division in tissue homeostasis

Adult stem cells continuously supply highly differentiated but short-lived cells, such as blood, skin, intestinal epithelium, and sperm cells, throughout life. To maintain the balance between stem cells and differentiating cells, a failure of which may lead to tumorigenesis through excess self-renewal or tissue degeneration through excess differentiation, many stem cells have the potential to divide asymmetrically so that each division produces one stem cell and one differentiating cell.

Drosophila male germ line stem cells (GSCs) serve as an ideal model system to study stem cell behavior. They reside in the stem cell niche, which specify stem cell identity by sending essential signal(s). Stem cells have elaborate cellular mechanisms to ensure the asymmetric outcome of the division, producing one stem cell and one differentiating cell, which is the key to tissue homeostasis.

I will present the latest discoveries on the mechanisms that ensure asymmetric outcome of the stem cell divisions.

Research LAB

Yukiko Yamashita Lab

Audience

Free and open to the university community and the public

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Butler Seminar Series Mon, 18 Aug 2014 15:09:18 -0400
The Art of Conversation http://molbio.princeton.edu/events/event/498-the-art-of-conversation http://molbio.princeton.edu/events/event/498-the-art-of-conversation Location: Lewis Thomas Lab, 005 - Princeton
Category: Mindfulness Workshop
Date: Thu, Feb 18, 2016 - Thu, Feb 18, 2016
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For faculty, staff, postdocs, and graduate students

The Art of Conversation

In today’s world, we turn away from each other, bent over computers, iPads, and phones, trying to connect online rather than with the people right in front of us. In this workshop, let’s discuss what we lose when we text instead of talk. Let’s reclaim the art of conversation and truly understand one another through the practice of mindfulness. Includes light lunch

Facilitated by Shefalika Gandhi, LCSW

http://uhs.princeton.edu/about-us/staff/shefalika-gandhi-lcsw

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Mindfulness Workshop Thu, 18 Dec 2014 14:07:07 -0500
Kush M. Parmar (5AM Ventures) http://molbio.princeton.edu/events/event/492-parmar http://molbio.princeton.edu/events/event/492-parmar Location: Schultz Lab, 107 - Princeton
Category: MOL BIO Translational Research Seminar
Date: Fri, Feb 19, 2016 - Fri, Feb 19, 2016
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MOL BIO Translational Research Seminar 

Speaker

Kush M. Parmar

Kush M. Parmar, M.D., Ph.D.
Managing Partner
5AM Ventures

 
Seminar Topic

Bridging two worlds: advancing academic science into biotech in the new age of biomedical innovation

The way medicines are discovered and developed has changed dramatically in the past decade, increasingly bringing the worlds of academia and industry far closer together in the pursuit of therapeutic advances. Industry’s eagerness to partner at earlier stages notwithstanding, key elements around a scientific breakthrough need to be in place to improve its prospects of ultimately being successful. Relevant examples of this new ecosystem will be shared through the lens of a Department of Molecular Biology alumnus who transitioned from academia to biotech venture capital and entrepreneurship. Importantly, as the world of biomedical innovation continues to evolve, so have the requirements and paths for academic discoveries to make their way into industry. Based on a few case studies and observations from the “front lines” of biotech entrepreneurship, some thoughts and advice for aspiring life sciences entrepreneurs will be shared.

Kush is a Managing Partner at 5 AM Ventures, a venture capital firm "dedicated to creating value in early-stage life science companies." Kush was in the Wieschaus lab as a Molecular Biology and Medieval Studies major at Princeton, and also holds a PhD in Experimental Pathology from Harvard University and an MD from Harvard Medical School. He previously served as Acting VP of Strategy and Corporate Development at Novira, and as Board Observer for Envoy Achaogen, and Pulmatrix. Kush recently joined the Advisory Council of the Department of Molecular Biology.

Audience

Free and open to the university community and the public.

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MOL BIO Translational Research Seminar Mon, 18 Aug 2014 15:09:18 -0400
Suzanne Eaton (Max Planck Institute) http://molbio.princeton.edu/events/event/467-eaton http://molbio.princeton.edu/events/event/467-eaton Location: Lewis Thomas Lab, 003 - Princeton
Category: Butler Seminar Series
Date: Wed, Feb 24, 2016 - Wed, Feb 24, 2016
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Butler Seminar Series

Speaker

Suzanne Eaton

Suzanne Eaton, Ph.D.
Research Group Leader
Max Planck Institute
Suzanne Eaton is a senior research group leader at the Max Planck Institute of Molecular Cell Biology and Genetics, and Professor at the Dresden Technical University. She obtained her PhD from UCLA studying immunoglobulin gene transcription.  She moved into developmental biology as a postdoc with Tom Kornberg at UCSF, where she worked on identifying the signaling circuits that set up the AP boundary organizer in Drosophila wing discs. To more deeply understand the cell biological mechanisms underlying epithelial development, she moved to the EMBL Cell Biology Programme for a second postdoc with Kai Simons. Her own research group (since 1999) has focused on the cell biological and biophysical mechanisms underlying development, encompassing topics that range from tissue mechanics through signaling and metabolism.
Seminar Topic

Mechanics of Drosophila pupal wing morphogenesis: an interplay between active and stress-induced cell dynamics

Research LAB

Suzanne Eaton Research

Audience

Free and open to the university community and the public

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Butler Seminar Series Mon, 18 Aug 2014 15:09:18 -0400
Andy Minn (Penn) http://molbio.princeton.edu/events/event/468-minn http://molbio.princeton.edu/events/event/468-minn Location: Lewis Thomas Lab, 003 - Princeton
Category: Butler Seminar Series
Date: Wed, Mar 02, 2016 - Wed, Mar 02, 2016
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Butler Seminar Series

Speaker

Photo of Andy Minn

Andy J. Minn, MD., Ph.D.
Assistant Professor of Radiation Oncology
University of Pennsylvania
Dr. Andy Minn is a board certified radiation oncologist and is affiliated with Abramson Family Cancer Research Institute at University of Pennsylvania in Philadelphia. He received his medical degree from University of Chicago Pritzker School of Medicine, then studied under Dr. Joan Massague at Sloan-Kettering Memorial Institute in New York City before returning to University of Chicago, Dept. of Radiation & Cellular Oncology, Ludwig Center for Metastasis Research as an Assistant Professor. He joined Univ. of Penn in 2010 and he is one of 39 doctors at Hospitals of the University of Pennsylvania-Penn Presbyterian and one of 24 at Penn Presbyterian Medical Center who specialize in Radiation Oncology.
Seminar Topic

Response and Resistance to Radiation and Immune Checkpoint Blockade for Cancer

My laboratory is focused on understanding how cancer acquires treatment resistance to both conventional therapies and to immunotherapies, and how resistance can be overcome. We have identified regulatory networks and signaling pathways that not only predict but also promote treatment resistance. These signaling pathways are normally associated with an anti-viral response, suggesting an intriguing overlap between anti-viral signaling and ways that cancer can evade the cytotoxic effects of therapy and/or the immune system. We are currently investigating this overlap by studying the regulation by the tumor microenvironment, how anti-viral responses are activated, and the mechanisms by which these pathways govern resistance. Studies using mouse models have been performed in parallel with analogous clinical trials in order to corroborate pre-clinical results with human patients 

Research 

Andy J. Minn Profile

Audience

Free and open to the university community and the public

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Butler Seminar Series Mon, 18 Aug 2014 15:09:18 -0400
Zhijian Chen (UT Southwestern Medical Center) http://molbio.princeton.edu/events/event/469-chen http://molbio.princeton.edu/events/event/469-chen Location: Lewis Thomas Lab, 003 - Princeton
Category: Butler Seminar Series
Date: Wed, Mar 09, 2016 - Wed, Mar 09, 2016
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Butler Seminar Series

Speaker

photo of Zjijian

Zhijian "James" Chen
Investigator, Howard Hughes Medical Institute
George L. MacGregor Distinguished Chair in Biomedical Science
Professor, Department of Molecular Biology
UT Southwestern Medical Center
Seminar Topic

TBA

Research 

Zhijian Chen Faculty Profile

Audience

Free and open to the university community and the public

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Butler Seminar Series Mon, 18 Aug 2014 15:09:18 -0400
Understanding Perfectionism http://molbio.princeton.edu/events/event/499-understanding-perfectionism http://molbio.princeton.edu/events/event/499-understanding-perfectionism Location: Lewis Thomas Lab, 005 - Princeton
Category: Mindfulness Workshop
Date: Thu, Mar 17, 2016 - Thu, Mar 17, 2016
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For faculty, staff, postdocs, and graduate students

Understanding Perfectionism

We’re bombarded by messages from the media that we must be the best, appear smart, act perfect and stay happy forever. Perfectionism is often mistakenly seen as necessary for success, but in reality perfectionist attitudes interfere with success and learning. In this mindfulness workshop, we will discuss some myths and realities of perfectionism, and reflect upon ways to be healthy strivers. 

Facilitated by Shefalika Gandhi, LCSW

http://uhs.princeton.edu/about-us/staff/shefalika-gandhi-lcsw

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Mindfulness Workshop Thu, 18 Dec 2014 14:07:07 -0500
Daniel Portnoy (UC, Berkeley) http://molbio.princeton.edu/events/event/470-portnoy http://molbio.princeton.edu/events/event/470-portnoy Location: Lewis Thomas Lab, 003 - Princeton
Category: Butler Seminar Series
Date: Wed, Mar 23, 2016 - Wed, Mar 23, 2016
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Butler Seminar Series

Speaker

Photo of Dan Portnoy

Daniel A. Portnoy
Professor, University of California, Berkeley
Dr. Portnoy received his undergraduate degree in bacteriology from UCLA in 1978 where his passion for microbiology.  He next earned his Ph.D. in 1983 under the tutelage of Stanley Falkow at the University of Washington and Stanford.  In the Falkow Lab, he worked on a conserved virulence plasmid in Yersinia enterocolitica, Y. pseudotuberculosis, and Y. pestis, and discovered what turned out to be the first effectors of type III secretion, though he incorrectly concluded that the “Yops” were outer-membrane proteins. To further his appreciation of host cells, he did his postdoctoral fellowship in the Zanvil Cohn Laboratory of Cellular Physiology and Immunology at the Rockefeller University in New York, working with Jay Unkeless and Jeff Ravetch.  At Rockefeller he worked on macrophage Fc receptors and lysosomal proteases. During a two-year stint at Washington University in St. Louis, he began working on Listeria monocytogenes as a model intracellular pathogen.  In 1988, he joined the Department of Microbiology at the University of Pennsylvania, where he collaborated with Lew Tilney in the Biology Department and made the observation that L. monocytogenes spreads from one cell to another by exploiting a host cell system of actin polymerization. His lab also defined the role of the listerial hemolysin in mediating dissolution of phagosomes. In collaboration with Phil Youngman, he showed that expression of the L. monocytogenes hemolysin by Bacillus subtilis led to its growth inside of host cells. Portnoy collaborated with Yvonne Paterson, who also arrived at Penn in 1988, on the use of L. monocytogenes as a recombinant vector-based vaccine for the induction of cell-mediated immunity. Both Paterson and Portnoy went on to work with biotech companies to develop vaccines for both cancer and infectious disease applications. Numerous clinical trials based on their discoveries have shown promising results as immunotherapeutic treatments for cancer. In 1997, Portnoy moved to UC Berkeley where he currently holds joint appointments in the Department of Molecular and Cell Biology and in the Division of Infectious Diseases and Vaccinology in the School of Public Health, and he was recently appointed as the Edward E. Penhoet Distinguished Chair in Global Public Health and Infectious Diseases.  While his lab continues to examine basic aspects of molecular and cell biology, the lab focus has moved into both innate and acquired immunity. Portnoy and collaborators have shown that immune cells recognize c-di-AMP, a novel and essential bacterial signaling molecule, secreted by L. monocytogenes through multidrug resistance efflux transporters.  Portnoy and Russell Vance identified that STING was the host receptor of cyclic-di-nucleotides (CDNs) that leading to the production of type I interferon and other co-regulated genes.  Modified CDNs are now being evaluated for clinical application as adjuvants and for cancer immunotherapy.  In 2013, Portnoy’s contributions were recognized by his election to the National Academy of Sciences.  
Seminar Topic

Cyclic-di-AMP: an essential Listeria monocytogenes signaling molecule that activates a host pathway of innate immunity

Prevention and treatment of diseases caused by intracellular pathogens remains one of the largest challenges facing the international biomedical community. A central problem that we address is how intracellular pathogens are recognized by the host and how the immune system integrates multiple signals to induce an appropriate response, and conversely, how pathogens avoid and/or manipulate the host response to promote their pathogenesis.  We have chosen to approach this problem by a detailed analysis of Listeria monocytogenes, an intracellular pathogen that has been studied for many decades as a model system with which to dissect basic aspects of infection & immunity.  In this seminar, I’ll discuss a genetic screen for bacterial mutants that induced elevated or diminished levels of a host innate immune response to infection that results in the host’s expression of IFN-b.  The results of the screen and subsequent biochemistry led to the discovery that L. monocytogenes secretes cyclic-di-AMP (CDA) through multidrug efflux pumps (MDRs) that activates a host protein called STING leading to the expression of IFN-b.  CDA turns out to be a conserved and essential small signaling molecule and I’ll discuss some of our recent results on its function in controlling bacterial metabolism. I’ll also mention some recent results using L. monocytogenes and CDA for cancer immunotherapies. 

Crimmins GT, Herskovits AA, Rehder K, Sivick KE, Lauer P, Dubensky TW Jr, and Portnoy DA. (2008). Listeria monocytogenes multidrug resistance transporters activate a cytosolic surveillance pathway of innate immunity. Proc Natl Acad Sci U S A. 2008 Jul 22;105(29):10191-6. Epub 2008 Jul 16. PMCID: PMC2481368

Woodward JJ, Iavarone AT, Portnoy DA. (2010). c-di-AMP secreted by intracellular Listeria monocytogenes activates a host type I interferon response. Science. 2010 Jun 25;328(5986): 1703-5. Epub 2010 May 27. PMCID: PMC3156580

Witte CE, Whiteley AT, Burke TP, Sauer, JD, Portnoy DA, Woodward JJ. (2013). Cyclic di-AMP is critical for Listeria monocytogenes growth, cell wall homeostasis, and establishment of infection. mBio. 2013 May 28;4(3). Doi:pii:e00282-13. 1128/mBio.00282-13. PMCID: PMC3663569

Archer KA, Durack J, and Portnoy DA. (2014). STING-dependent Type I IFN production inhibits cell-mediated immunity to Listeria monocytogenes.  PLoS Pathog. 2014 Jan;10(1):e1003861. doi: 10.1371/journal.ppat.1003861. Epub 2014 Jan 2. PMCID: PMC3879373

Whiteley AT, Pollock AJ, Portnoy DA.  (2015). The PAMP c-di-AMP is essential for Listeria growth in macrophages and rich but not minimal media, due to a toxic increase in (p)ppGpp. Cell Host Microbe. 2015 Jun 10;17(6):788-98. doi: 10.1016/j.chom.2015.05.006. Epub 2015 May 28.  PMCID: PMC4469362

Research 

The Portnoy Lab

Audience

Free and open to the university community and the public

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Butler Seminar Series Mon, 18 Aug 2014 15:09:18 -0400
Anne Brunet (Stanford U) http://molbio.princeton.edu/events/event/471-brunet http://molbio.princeton.edu/events/event/471-brunet Location: Lewis Thomas Lab, 003 - Princeton
Category: Butler Seminar Series
Date: Wed, Mar 30, 2016 - Wed, Mar 30, 2016
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Butler Seminar Series

Speaker

Anne Brunet

Anne Brunet
Professor of Genetics
Stanford School of Medicine
Seminar Topic

TBA

Research 

The Brunet Lab

Audience

Free and open to the university community and the public

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Butler Seminar Series Mon, 18 Aug 2014 15:09:18 -0400
Kenneth Yamada (NIH) http://molbio.princeton.edu/events/event/472-yamada http://molbio.princeton.edu/events/event/472-yamada Location: Lewis Thomas Lab, 003 - Princeton
Category: Butler Seminar Series
Date: Wed, Apr 06, 2016 - Wed, Apr 06, 2016
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Butler Seminar Series

Speaker

Photo of Ken Yamada

Kenneth Yamada, MD., Ph.D.
NIH Distinguished Investigator 
Chief, Laboratory of Cell and Developmental Biology
Head, Cell Biology Section
National Institutes of Health/NIDCR 
Seminar Topic

TBA

Research 

Kenneth Yamada Profile

Audience

Free and open to the university community and the public

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Butler Seminar Series Mon, 18 Aug 2014 15:09:18 -0400