Princeton University Molecular Biology - Molecular Biology Events Mon, 20 Oct 2014 01:11:15 -0400 Joomla! - Open Source Content Management en-gb Lori Passmore (MRC Laboratory of Molecular Biology, Cambridge) Location: Schultz Lab, 107 - Princeton
Category: Special Seminar
Date: Mon, Oct 20, 2014 - Mon, Oct 20, 2014
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Special Seminar

Lori Passmore
Lori Passmore, Ph.D.
MRC Laboratory of Molecular Biology,

Lori Passmore is a Group Leader at the MRC Laboratory of Molecular Biology (LMB) in Cambridge UK. She received her BSc in Biochemistry from the University of British Columbia in Vancouver and her PhD from The Institute of Cancer Research in London UK where she worked with Prof David Barford. Lori was a Beit Postdoctoral Fellow in the lab of Venki Ramakrishnan at MRC LMB where she studied the structures of eukaryotic ribosomes. Lori started her own lab in 2009 and studies the molecular mechanisms of multi-protein complexes that regulate gene expression.

Seminar Topic

Structural insights into macromolecular machines that regulate mRNA polyA tails

Almost all eukaryotic mRNAs have a 3´ polyA tail that contributes to post-transcriptional regulation of gene expression. PolyA tails influence both translation and mRNA stability. My laboratory studies the macromolecular protein complexes that control addition and removal of poly(A) tails. We use a hybrid approach combining structural (cryo-EM, x-ray crystallography, NMR), biochemical, biophysical and genetic techniques to gain insights into their molecular mechanisms. We recently purified and reconstituted the activity of the conserved eukaryotic Pan2–Pan3 deadenylation complex. I will discuss structural and mechanistic studies that have provided insights into the function of Pan2–Pan3, including mRNA recruitment. In addition, I will discuss recent advances in sample preparation for electron cryo-microscopy (cryo-EM) that we are using to study these complexes.

Research lab


Free and open to the university community and the public

Special Seminar Mon, 18 Aug 2014 15:09:18 -0400