Core Facilities
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Histology Core Facility Histology

Contact

Histology Core Facility
Department of Molecular Biology
Princeton University
113B Moffett Laboratory
Washington Rd
Princeton, NJ 08544

T (609) 258-7026 | F (609) 258-8468

Overview of Histology Core Facility

The Molecular Biology Histology facility maintains automatic tissue processor, tissue embedding center, rotary microtome, cryostat, and supplemental equipment. The Histology facility provides hands-on training of sample preparation for Histology and Immunohistochemistry with paraffin embedded or optimal cutting temperature (OCT) compound embedded sample as well as operation training of Histology equipment. Trained users will be able to better understand histology and immunohistochemistry for their study.

Pricing

Category Items Price/UOM
Histology Sectioning equipment $12.60/cost

User Training

Sample preparation of histology and immunohistochemistry

Operation of Histology equipment

Histology Equipment

Rotary Microtome (Leica RM2255)

Leica RM2255 Rotary Microtome

This microtome is used to section tissue that has been fixed and embedded in either paraffin or resin. This type of sectioning is required when maintaining proper morphology of the tissue is critical. The microtome will enable to cut sections 0.5um to 100um in thickness. The sections are needed for small tissue including, but not limited to sectioning of zebrafish embryo. The ability to also do larger sections will make this useful for mouse brain slices and others.

Cryostat (Leica CM3050)

Leica CM3050S Cryostat

This cryostat is used for sectioning of frozen tissue sample. Benefits of cryosection are that not only its sample preparation time is shorter than paraffin sample preparation, but also the cryosection can be used for immunohistochemistry or in situ analysis with better antigenicity. This cryostat has the ability to section 0.5μm to 300μm in thickness and can be cooled down to -40°C.

Tiss Processor (Leica ASP300S)

Leica ASP 300S Automated Tissue Processor

This tissue processor provides automatic formalin-fixation, EtOH-dehydration, and paraffin-infiltration of tissue sample(s).

Paraffin Embedding Center (Tiss TEK)

Miles Scientific Tissue-TEK Tissue Embedding Center

This allows the tissue sample to be embedded into mold with paraffin to make paraffin block.

Glass Knife Maker (RMC)

Glass Knife Maker

This knife maker provides a glass knife for semi-thin and ultra-thin sectioning with ultramicrotome.

MolBio Events

Wed, Apr 23, 2014

Thu, Apr 24, 2014

Fri, Apr 25, 2014

Tue, Apr 29, 2014

Contact Us

Lewis Thomas Laboratory at Princeton University

119 Lewis Thomas Laboratory
Washington Road, Princeton, NJ  08544-1014

Tel: (609) 258-3658
Fax: (609) 258-3980
Website:  molbio.princeton.edu