Laser scanning confocal microscopy provides our researchers the ability to optically section whole specimens of cells and small organisms, such as developing fly and fish embryos, or very thick sections from brain and other organ tissues that have been tagged with fluorescent probes. This technology provides us with the highest light microscope resolution obtainable, giving the scientist a clearer picture of sub cellular structures, function, and cellular or organism architecture. We can perform this on single or multiply labeled specimens. Our laboratory has 20 years experience of confocal imaging in mammalian cells, yeast cells, Drosophila and Zebra fish embryos and ovaries, bacteria, sections of brain and other tissues, in both fixed and live specimens. Quantitative methods such as FRAP, FLIP AND FRET are available. The laboratory has several software packages for image analysis, processing quantitation and 3D image reconstruction.