Confocal Microscopy

Instruments

	Nikon A1 and Nikon A1-RS Confocal Microscopes Nikon A1 –RS this is the same scope as the Nikon A-1 except it has 2 detector systems and 2 scanning system. Both systems can collect images from 64 X 64 pixels up to 4096 X 4096 pixels Lasers Lines at 405, 488, 560, and 642 nms. The 405,560 and 642 are solid state lasers. The 488 laser is a 50mWatt gas laser. Detectors: 4 Fluorescent PMT’s and 1 Transmitted Detector Both Systems are mounted on Nikon Eclipse TI microscopes. Accessories: Motorized XY Stage and Piezo Z stage for rapid sectioning. Stage top incubator for temperature and CO2 atmospheric control Vibration Isolation tables from Newport Corporation 30” X 48 “. Nikon A1-RS Scanners: 1 Pair of Galvo XY scanners 1 Resonant Scanner: Collects 25 FPS at 512 x 512 pixels
	Leica sp5 5 channel spectral imaging system with excitations at 405, 458, 476, 488, 514, 561, 594 and 633. Spectral seperation of overlapping fluorophores Imaging sppeds from 10 to 1,400 hertz with mirror scanning Resonant scanning at 8,000 hertz [25 frames / second at 512 x 512 pixel resolution]. Fast z- stepping with galvo z stage Live cell imaging at controlled temperatures employing warner heating unit Frap, fret , flip and visible uncaging
	Perkinelmer spinning disk confocal This confocal microscope was installed in August 2005. We recently upgraded to 3 solid state lasers, a 50mWatt 488, a 50mWatt 560 and a 40 mWatt 640 providing 3 channel imaging plus transmitted light. We also just added a 100 mWatt 285 Micropoint laser system that can produce a 365 nm line for ablation, 405 nm line for photo-activation or photo-conversion and a 4354 nm line for photo-bleaching to perform FRAP experiments. System is located in Moffett 121.
	Prairie ultima multi-photon system 2 channel imaging using 2 GaAsp detectors per channel. A Coherent Chameleon Ultima II pulse laser, tunable from 680 to 1080 nms. Dual scanning system - galvo scanners and an AOD scanner with scan speeds up to 25 fps at 512 x 512 resolution Fast Prior piezo z-stage with 500 microns of travel 405 laser for ablation, photo-activation and photo-conversion Environmental chamber for live cell mammalian imaging. Temp. Range 25 to 45 degrees C.\ Objective lenses: 1.0 NA20 x and 40 x Plan Apo's, 1.3 NA 40 x Plan Apo 0.2 NA 5X Plan fluor and 10 x 0.6 NA Plan Apo