Nikon A1 and Nikon A1-RS Confocal Microscopes
At the heart of the Nikon A1 hybrid confocal scan head is the dual scanning system that encompass a standard-speed scanner and a high-speed resonant scanner. They may be used individually or simultaneously. Applications include:
For the standard speed scanner: imaging of all fixed specimens and many live specimens, including FRET and ion ratio imaging (e.g.: calcium, pH);
High speed resonant scanner: Maximum frame rate can exceed 400 frames per second.
Simultaneous use of standard and resonant scanners: Useful for FRAP and photoactivation specimens that require stimulating with one scanner while simultaneously imaging with the other.
Three Detector Ports are Standard
405nm diode laser, 561nm diode-pumped-solid-state (DPSS) laser, and a 638nm diode laser.
An argon-ion gas laser is the fourth laser. It produces four lines: 457nm, 477nm, 488nm, and 514nm line.
Nikon Perfect Focus System (PFS) is the solution to keep cells in focus over prolonged time-lapse imaging sessions. Pick the focal plane and PFS monitors the focus every 5ms to maintain focus indefinitely.
Accessories: Motorized XY Stage and Piezo Z stage for rapid sectioning.
The Leica SP5 Confocal has a filter-free spectral detector system with five individually tunable channels. The spectral separation is accomplished by a unique prism design.
Each detector is freely tunable over the entire visible spectrum. The SP5 has a prism base beam splitter (AOBS) that allows for free selection of up to 8 Lasers from UV – IR. The spectral range of the detector optics covers 400 - 800nm.
The scanner can be run in either conventional or resonant modes. The conventional scanner can be scanned unidirectional or bi-directional with a speeds ranging from 10Hz to 2800Hz. The resonant scan can also be scanned unidirectional or bi-directional at 8000Hz or 1600Hz respectively with a maximum frame rate of 250.
The system is equipped with a Z-galvanometer that allow for XZ scanning as well as fast XYZ scanning. The scanning stage allows for stitching together images from large fields of view as well as imaging multiple positions as XYZT images.
The laser lines available on the SP5 are 405, 458, 476, 488, 514, 561, 594 and 633. Of the 5 detectors on the system 3 are conventional PMTs, 2 are Hybrid detectors (HyD). Leica HyD detectors provide highly sensitive photon detection.
There is a Warner chamber for temperature control of live experiments.
Perkinelmer spinning disk confocal
This confocal microscope was installed in August 2005. We recently upgraded to 3 solid state lasers, a 50mWatt 488, a 50mWatt 560 and a 40 mWatt 640 providing 3 channel imaging plus transmitted light. We also just added a 100 mWatt 285 Micropoint laser system that can produce a 365 nm line for ablation, 405 nm line for photo-activation or photo-conversion and a 4354 nm line for photo-bleaching to perform FRAP experiments. System is located in Moffett 121.
Prairie ultima multi-photon system
2 channel imaging using 2 GaAsp detectors per channel. A Coherent Chameleon Ultima II pulse laser, tunable from 680 to 1080 nms.
Dual scanning system - galvo scanners and an
AOD scanner with scan speeds up to 25 fps at 512 x 512 resolution
Fast Prior piezo z-stage with 500 microns of travel
405 laser for ablation, photo-activation and photo-conversion
Environmental chamber for live cell mammalian imaging. Temp. Range 25 to 45 degrees C.\
Objective lenses: 1.0 NA20 x and 40 x Plan Apo's, 1.3 NA 40 x Plan Apo
0.2 NA 5X Plan fluor and 10 x 0.6 NA Plan Apo