Instruments

The facility is currently equipped with the following instrumentation

Flow Cytometer

BD LSRII Multi-Laser Analyzer w/ HTS 

  • Five-laser fixed alignment benchtop system. 
  • 355, 405, 488, 561, & 640nm lasers for excitation. 
  • Capable of up to 14-color acquisition. 
  • Equipped with high-throughput 96 & 384-well plate automated microsampler.

Cell Sorters

BD FACSVantage SE w/ DiVa 

Three laser cell sorting system with advanced digital acquisition (DiVa) technology. 

  • Tunable laser excitation sources. Ultraviolet, 488, 568 & 647nm lines are most commonly utilized. 
  • Capable of up to 10 parameter (8-color, 2-scatter) acquisition. 
  • Performs sorting into multi-well plates or four separate tubes. 
  • Equipped with sample and sort tube temperature control.

Bio-Rad S3 Cell Sorter 

Dual laser (488 & 561nm) benchtop cell sorter. • 

  • Capable of 6 parameter (4-color, 2-scatter) detection. 
  • Automated setup: has automated startup, stream-to-laser alignment and drop delay calculation. 
  • Automated operation monitoring: has droplet breakoff , sample volume, and sort collection tube volume monitoring for walk-away sorting. 
  • Automated fluidics monitoring: all reservoirs are self-monitoring and display the amount of time before a swap is required. 
  • Fluids may be ‘hot-swapped’ during a sort. 
  • Temperature control of the sort and sample tube holders. 
  • Available to trained users 24/7.

COPAS BioSort Worm Sorter 

 Large particle cytometer for analyzing, sorting and dispensing cells and organisms 40-175µm in size. 

  • Two laser excitation lines: 488 & 514nm with five parameter (3-color) acquisition. 
  • ReFlx system for sampling directly from 96-well plates. 
  • Profiler II option that allows for the spatial mapping of fluorescence signals along the length of the organism.

Particle Counter

Beckman Coulter Z2 Particle Counter 

  • 100µm aperture for particle sizing and counting via Coulter Principle (Electrical Sensing Zone Method). 
  • Useful for yeast and mammalian cell counts. 
  • Reports count, concentration, and size distribution results. 
  • Equipped with AccuComp software for improved data analysis and presentation. 

Centrifuge 

Cytospin

  • Thin-layer cell preparation system. 
  • Uses centrifugation to deposit cells onto a clearly defined area of a glass slide. 
  • Can process up to 12 specimens at one time.